Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures
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http://hdl.handle.net/10045/67477
Títol: | Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures |
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Autors: | Hidalgo, Diego | Abdoli-Nasab, Maryam | Jalali-Javaran, Mokhtar | Bru-Martinez, Roque | Cusidó, Rosa M. | Corchete, Purificación | Palazon, Javier |
Grups d'investigació o GITE: | Proteómica y Genómica Funcional de Plantas |
Centre, Departament o Servei: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Paraules clau: | Tobacco cell cultures | Transplastomic plants | Human tissue plasminogen activator (tPA) | Reteplase |
Àrees de coneixement: | Bioquímica y Biología Molecular |
Data de publicació: | de setembre-2017 |
Editor: | Elsevier |
Citació bibliogràfica: | Plant Physiology and Biochemistry. 2017, 118: 130-137. doi:10.1016/j.plaphy.2017.06.013 |
Resum: | Transplastomic plants are a system of choice for the mass production of biopharmaceuticals due to the polyploidy of the plastid genome and the low risk of pollen-mediated outcrossing because of maternal inheritance. However, as field-grown plants, they can suffer contamination by agrochemicals and fertilizers, as well as fluctuations in yield due to climatic changes and infections. Tissue-type plasminogen activator (tPA), a protein used to treat heart attacks, converts plasminogen into plasmine, which digests fibrin and induces the dissolution of fibrin clots. Recently, we obtained transplastomic tobacco plants carrying the K2S gene encoding truncated human tPA (reteplase) with improved biological activity, and confirmed the presence of the target protein in the transgenic plant leaves. Considering the advantages of plant cell cultures for biopharmaceutical production, we established a cell line derived from the K2S tobacco plants. The active form of reteplase was quantified in cultures grown in light or darkness, with production 3-fold higher in light. |
Patrocinadors: | This work has been supported by a grant from the Spanish Ministry of Science and Innovation (BIO2014-51861-R) and the Generalitat de Catalunya (2014SGR215). Diego Hidalgo is a predoctoral fellow of Mexican CONACyT. |
URI: | http://hdl.handle.net/10045/67477 |
ISSN: | 0981-9428 (Print) | 1873-2690 (Online) |
DOI: | 10.1016/j.plaphy.2017.06.013 |
Idioma: | eng |
Tipus: | info:eu-repo/semantics/article |
Drets: | © 2017 Elsevier Masson SAS. |
Revisió científica: | si |
Versió de l'editor: | http://dx.doi.org/10.1016/j.plaphy.2017.06.013 |
Apareix a la col·lecció: | INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas |
Arxius per aquest ítem:
Arxiu | Descripció | Tamany | Format | |
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2017_Hidalgo_etal_PlantPhyBiochem_final.pdf | Versión final (acceso restringido) | 2,58 MB | Adobe PDF | Obrir Sol·licitar una còpia |
2017_Hidalgo_etal_PlantPhyBiochem_accepted.pdf | Accepted Manuscript (acceso abierto) | 925,96 kB | Adobe PDF | Obrir Vista prèvia |
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