Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli
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http://hdl.handle.net/10045/33397
Títol: | Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli |
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Autors: | Almendros, Cristóbal | Guzmán, Noemí M. | Díez-Villaseñor, César | García-Martínez, Jesús | Mojica, Francisco J.M. |
Grups d'investigació o GITE: | Microbiología Molecular |
Centre, Departament o Servei: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Paraules clau: | CRISPR-Cas systems | Protospacer adjacent motif | Natural immunity | Escherichia coli |
Àrees de coneixement: | Microbiología |
Data de publicació: | 26-de novembre-2012 |
Editor: | Public Library of Science (PLoS) |
Citació bibliogràfica: | ALMENDROS, Cristóbal, et al. “Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli”. PLoS ONE 7(11): e50797. doi:10.1371/journal.pone.0050797 |
Resum: | Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (cas) genes conform the CRISPR-Cas systems of various bacteria and archaea and produce degradation of invading nucleic acids containing sequences (protospacers) that are complementary to repeat intervening spacers. It has been demonstrated that the base sequence identity of a protospacer with the cognate spacer and the presence of a protospacer adjacent motif (PAM) influence CRISPR-mediated interference efficiency. By using an original transformation assay with plasmids targeted by a resident spacer here we show that natural CRISPR-mediated immunity against invading DNA occurs in wild type Escherichia coli. Unexpectedly, the strongest activity is observed with protospacer adjoining nucleotides (interference motifs) that differ from the PAM both in sequence and location. Hence, our results document for the first time native CRISPR activity in E. coli and demonstrate that positions next to the PAM in invading DNA influence their recognition and degradation by these prokaryotic immune systems. |
Patrocinadors: | This study was supported by the Spanish Ministerio de Ciencia e Innovación (BIO2011-24417). |
URI: | http://hdl.handle.net/10045/33397 |
ISSN: | 1932-6203 |
DOI: | 10.1371/journal.pone.0050797 |
Idioma: | eng |
Tipus: | info:eu-repo/semantics/article |
Drets: | © 2012 Almendros et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Revisió científica: | si |
Versió de l'editor: | http://dx.doi.org/10.1371/journal.pone.0050797 |
Apareix a la col·lecció: | INV - Microbiología Molecular - Artículos de Revistas |
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