Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers
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Título: | Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers |
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Autor/es: | Taïbi, Khaled | Campo, Antonio D. del | Vilagrosa, Alberto | Bellés, José M. | López-Gresa, María Pilar | Pla, Davinia | Calvete, Juan J. | López-Nicolás, José M. | Mulet, José M. |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ecología | CEAM (Centro Estudios Ambientales del Mediterráneo) |
Palabras clave: | Pinus halepensis | Aleppo pine | Drought tolerance | Physiological response | Soluble sugars | Free amino acids | Plant proteomics | Glutathione |
Área/s de conocimiento: | Ecología |
Fecha de publicación: | 24-jul-2017 |
Editor: | Frontiers Media |
Cita bibliográfica: | Taïbi K, del Campo AD, Vilagrosa A, Bellés JM, López-Gresa MP, Pla D, Calvete JJ, López-Nicolás JM and Mulet JM (2017) Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers. Front. Plant Sci. 8:1202. doi: 10.3389/fpls.2017.01202 |
Resumen: | Drought is one of the main constraints determining forest species growth, survival and productivity, and therefore one of the main limitations for reforestation or afforestation. The aim of this study is to characterize the drought response at the physiological and molecular level of different Pinus halepensis (common name Aleppo pine) seed sources, previously characterized in field trials as drought-sensitive or drought-tolerant. This approach aims to identify different traits capable of predicting the ability of formerly uncharacterized seedlings to cope with drought stress. Gas-exchange, water potential, photosynthetic pigments, soluble sugars, free amino acids, glutathione and proteomic analyses were carried out on control and drought-stressed seedlings in greenhouse conditions. Gas-exchange determinations were also assessed in field-planted seedlings in order to validate the greenhouse experimental conditions. Drought-tolerant seed sources presented higher values of photosynthetic rates, water use efficiency, photosynthetic pigments and soluble carbohydrates concentrations. We observed the same pattern of variation of photosynthesis rate and maximal efficiency of PSII in field. Interestingly drought-tolerant seed sources exhibited increased levels of glutathione, methionine and cysteine. The proteomic profile of drought tolerant seedlings identified two heat shock proteins and an enzyme related to methionine biosynthesis that were not present in drought sensitive seedlings, pointing to the synthesis of sulfur amino acids as a limiting factor for drought tolerance in Pinus halepensis. Our results established physiological and molecular traits useful as distinctive markers to predict drought tolerance in Pinus halepensis provenances that could be reliably used in reforestation programs in drought prone areas. |
Patrocinador/es: | This study is a part of the research project: “Application of molecular biology techniques in forest restoration in Mediterranean environments, PAID-05-11” funded by the Universitat Politècnica de València (UPV), program for supporting R&D of new multidisciplinary research lines. The authors are grateful to the Ministerio de Economía y Competitividad AGL2014-57431-P. AV was supported by project Survive-2 (CGL2015-69773-C2-2-P MINECO/FEDER) by the Spanish Government and Prometeo program (DESESTRES-Generalitat Valenciana). CEAM is funded by Generalitat Valenciana. |
URI: | http://hdl.handle.net/10045/68456 |
ISSN: | 1664-462X |
DOI: | 10.3389/fpls.2017.01202 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2017 Taïbi, del Campo, Vilagrosa, Bellés, López-Gresa, Pla, Calvete, López-Nicolás and Mulet. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.3389/fpls.2017.01202 |
Aparece en las colecciones: | Personal Investigador sin Adscripción a Grupo INV - GEB - Artículos de Revistas |
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