Molecular response of cocoa (Theobroma cacao) to water deficit conditions
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Título: | Molecular response of cocoa (Theobroma cacao) to water deficit conditions |
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Autor/es: | Montenegro, J. | Morante Carriel, Jaime | Acosta-Farías, Milena | Jaimez, R. | Carranza Patiño, Mercedes | Bru-Martinez, Roque | Huebla-Concha, Víctor | Morante, L. | Abasolo, F. | Sepulveda, N. | Quiñones, John |
Grupo/s de investigación o GITE: | Proteómica y Genómica Funcional de Plantas |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Bioquímica y Biología Molecular y Edafología y Química Agrícola | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Palabras clave: | Genes | Hybridization | Rootstock | Grafts | Water deficit | Local Species |
Fecha de publicación: | 20-ago-2023 |
Editor: | Pakistan Agricultural Scientists Forum |
Cita bibliográfica: | Journal of Animal and Plant Sciences. 2023, 33(6): 1314-1321. https://doi.org/10.36899/JAPS.2023.6.0671 |
Resumen: | Theobroma cacao is very important in the economy of many tropical countries, especially in Ecuador. The development and production of Theobroma cacao have been limited by the attack of diseases, pests, and currently by the impact caused by the water deficit. The loss of water in the crop causes a low production of the plantations. In addition, when genetic diversity is scarce, the possible combinations of genes capable of conferring biological adaptation and, consequently, the capacity to adapt to environmental variations, are reduced. Therefore, a viable option is to identify genes capable of conferring adaptation to water deficit stress. The objective of this research was to characterize genes of Theobroma cacao in response to water deficit. The sequencing and characterization of genes related to water stress were achieved and, finally, the hybridization profile of the genes (ABA, ODC, DEH, and ABS) in response to Actin (ACT) was analyzed. The ABS gene hybridized 100 % with DNA from rootstock EET399 in roots and 80 % with DNA from graft T24 in leaves. The OCD gene hybridized 80 % with DNA from rootstock IMC6 and 25 % with the T800 graft in leaves. The DEH gene hybridized below the normalizing gene (ACT) in both rootstock EET400 (10 %) in root DNA and graft T12 (5 %) in leaves. Finally, the ABA gene did not hybridize with the DNA of the CCN51 rootstock or with the graft (T801) in both roots and leaves, respectively. This research is key to understand some molecular mechanisms of T. cacao in response to water deficit and can be considered as a viable strategy to reduce crop agronomic losses under the current environmental scenario. |
Patrocinador/es: | The authors thanks to the Competitive Funds for Scientific and Technological Research (FOCICYT, Seventh Call 2018), to the Faculty of Forestry and Agricultural Sciences, to the Deanship of Scientific Research at the State Technical University of Quevedo, Ecuador and the Department of Biochemistry and Molecular Biology a Edaphology and Agricultural Chemistry at the University of Alicante. Spain. |
URI: | http://hdl.handle.net/10045/139509 |
ISSN: | 1018-7081 (Print) | 2309-8694 (Online) |
DOI: | 10.36899/JAPS.2023.6.0671 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.36899/JAPS.2023.6.0671 |
Aparece en las colecciones: | INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas |
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Montenegro_etal_2023_JAnimPlantSci.pdf | 1,22 MB | Adobe PDF | Abrir Vista previa | |
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