A new centrifugation/filtration technique to improve the observation of calcareous nannofossils under the scanning electron microscope
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Title: | A new centrifugation/filtration technique to improve the observation of calcareous nannofossils under the scanning electron microscope |
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Authors: | Lancis Sáez, Carlos | Tent-Manclus, Jose Enrique | Flores, José Abel |
Research Group/s: | Evolución Geodinámica de la Cordillera Bética Oriental y de la Plataforma Marina de Alicante |
Center, Department or Service: | Universidad de Alicante. Departamento de Ciencias de la Tierra y del Medio Ambiente |
Keywords: | Calcareous nannofossils | Preparation technique | Filtration technique | Scanning electron microscope |
Issue Date: | Apr-2023 |
Publisher: | International Nannoplankton Association |
Citation: | Journal of Nannoplankton Research. 2023, 41(1): 33-39 |
Abstract: | A new sediment sample preparation procedure for observing calcareous nannofossils using a scanning electron microscope (SEM) is described. The new preparation technique is a moderately time-consuming technique that allows observation of the finest structural details of specimens in the SEM. It has two separate steps: 1) disaggregation employing H2O2 and an ultrasound bath, keeping the pH stable through the use of a NaCO3-HCO3 buffer solution; and 2) a filtration process that concentrates and cleans the nannofossils through use of a vacuum pump. To do this, previous techniques for improving the quality of SEM images were combined, aimed at avoiding dissolution in the process, for which a slow digestion of the organic matter was carried out using a buffered solution, adding centrifugation and controlled vacuum pumping in order to retain the coccoliths without affecting their preservation. |
Sponsor: | This work was supported by Projects RTI2018-099489-B-I00 and PID2020-114381GB-I00, granted by the Spanish Ministry of Science and Innovation, and also by the European Union Next-GenerationEU Project GVA-THINKINAZUL/2021/039. |
URI: | http://hdl.handle.net/10045/135107 |
ISSN: | 1210-8049 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2023 International Nannoplankton Association |
Peer Review: | si |
Publisher version: | http://ina.tmsoc.org/JNR/JNRcontents.htm |
Appears in Collections: | INV - GEODIN - Artículos de Revistas |
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