A New Advanced and Validated Method for the Determination of Potentially Toxic Metals and Trace and Ultra-Trace Elements in Peritoneal Fluid to Assess the Health Risks of Women with Gynecological Pathologies
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/133902
Título: | A New Advanced and Validated Method for the Determination of Potentially Toxic Metals and Trace and Ultra-Trace Elements in Peritoneal Fluid to Assess the Health Risks of Women with Gynecological Pathologies |
---|---|
Autor/es: | López-Botella, Andrea | Sánchez, Raquel | Todolí Torró, José Luis | Gómez-Torres, María José | Velasco-Ruiz, Irene | Acién, Maribel |
Grupo/s de investigación o GITE: | Análisis de Polímeros y Nanomateriales | Grupo de Inmunología, Biología Celular y del Desarrollo |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología | Universidad de Alicante. Departamento de Biotecnología |
Palabras clave: | ICP-MS/MS | Potentially toxic elements | Peritoneal fluid | Gynecological pathologies | Health effects | Environmental contamination |
Fecha de publicación: | 23-abr-2023 |
Editor: | MDPI |
Cita bibliográfica: | López-Botella A, Sánchez R, Todolí-Torró J-L, Gómez-Torres MJ, Velasco I, Acién M. A New Advanced and Validated Method for the Determination of Potentially Toxic Metals and Trace and Ultra-Trace Elements in Peritoneal Fluid to Assess the Health Risks of Women with Gynecological Pathologies. Toxics. 2023; 11(5):399. https://doi.org/10.3390/toxics11050399 |
Resumen: | Toxic metals found in the environment have been linked to female infertility and gynecological illnesses. Reliable analytical methods, such as inductively coupled plasma tandem mass spectrometry (ICP-MS/MS), are necessary to determine the elemental composition of biological samples. Currently, the multielemental profile of peritoneal fluid (PF) samples has not yet been established. Due to the complexity of the PF matrix, an ICP-MS/MS-based method has been optimized to mitigate matrix effects and spectral interferences. A dilution factor of 1:4 was the best option to mitigate matrix effects while keeping sensitivity at an appropriate level. A collision gas (He) was useful to lower the extent of spectral interferences for 56Fe, 52Cr, 63Cu, and 68Zn. An intermediate validation test was performed to evaluate accuracy, achieving recoveries ranging from 90 to 110%. The method was validated in terms of intermediate precision, reproducibility, and trueness, with an expanded uncertainty lower than 15%. Afterward, it was applied to perform the multielemental analysis of 20 PF samples. The concentrations for major analytes were up to 151 µg L−1. Meanwhile,209Bi, 111Cd, 52Cr, 55Mn, 95Mo, 60Ni, 208Pb, 118Sn, and 51V were present at concentrations included within the 1–10 µg L−1 range, while 59Co and 139La were found at concentrations below 1 µg L−1. |
Patrocinador/es: | This study was funded by the Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (UGP-19-042 and UGP-20-039). A.L.-B. is supported by the European Social Fund and the Generalitat Valenciana under a Ph.D. contract (ACIF–2021/243). |
URI: | http://hdl.handle.net/10045/133902 |
ISSN: | 2305-6304 |
DOI: | 10.3390/toxics11050399 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/toxics11050399 |
Aparece en las colecciones: | INV - NANOBIOPOL - Artículos de Revistas INV - Grupo de Inmunología - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Lopez-Botella_etal_2023_Toxics.pdf | 1,18 MB | Adobe PDF | Abrir Vista previa | |
Este ítem está licenciado bajo Licencia Creative Commons