The puzzling mitochondrial phylogeography of the black soldier fly (Hermetia illucens), the commercially most important insect protein species
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Título: | The puzzling mitochondrial phylogeography of the black soldier fly (Hermetia illucens), the commercially most important insect protein species |
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Autor/es: | Ståhls, Gunilla | Meier, Rudolf | Sandrock, Christoph | Hauser, Martin | Zorić, Ljiljana Šašić | Laiho, Elina | Aracil, Andrea | Doderović, Jovana | Badenhorst, Rozane | Unadirekkul, Phira | Adom, Nur Arina Binte Mohd | Wein, Leo | Richards, Cameron | Tomberlin, Jeffery K. | Rojo, Santos | Veselić, Sanja | Parviainen, Tuure |
Grupo/s de investigación o GITE: | Bionomía, Sistemática e Investigación Aplicada de Insectos Dípteros e Himenópteros |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias Ambientales y Recursos Naturales |
Palabras clave: | Black soldier fly | Hermetia illucens | mtDNA COI haplotypes | Nuclear rDNA |
Área/s de conocimiento: | Zoología |
Fecha de publicación: | 24-may-2020 |
Editor: | BioMed Central |
Cita bibliográfica: | BMC Evolutionary Biology. 2020, 20:60. doi:10.1186/s12862-020-01627-2 |
Resumen: | Background: The black soldier fly (Diptera: Stratiomyidae, Hermetia illucens) is renowned for its bioconversion ability of organic matter, and is the worldwide most widely used source of insect protein. Despite varying extensively in morphology, it is widely assumed that all black soldier flies belong to the same species, Hermetia illucens. We here screened about 600 field-collected and cultured flies from 39 countries and six biogeographic regions to test this assumption based on data for three genes (mitochondrial COI, nuclear ITS2 & 28S rDNA) and in order to gain insights into the phylogeography of the species. Results: Our study reveals a surprisingly high level of intraspecific genetic diversity for the mitochondrial barcoding gene COI (divergences up to 4.9%). This level of variability is often associated with the presence of multiple species, but tested nuclear markers (ITS2 and 28S rDNA) were invariant and fly strain hybridization experiments under laboratory conditions revealed reproductive compatibility. COI haplotype diversity is not only very high in all biogeographic regions (56 distinct haplotypes in total), but also in breeding facilities and research centers from six continents (10 haplotypes: divergences up to 4.3%). The high genetic diversity in fly-breeding facilities is mostly likely due to many independent acquisitions of cultures via sharing and/or establishing new colonies from field-collected flies. However, explaining some of the observed diversity in several biogeographic regions is difficult given that the origin of the species is considered to be New World (32 distinct haplotypes) and one would expect severely reduced genetic diversity in the putatively non-native populations in the remaining biogeographic regions. However, distinct, private haplotypes are known from the Australasian (N = 1), Oriental (N = 4), and the Eastern Palearctic (N = 4) populations. We reviewed museum specimen records and conclude that the evidence for introductions is strong for the Western Palearctic and Afrotropical regions which lack distinct, private haplotypes. Conclusions: Based on the results of this paper, we urge the black soldier fly community to apply molecular characterization (genotyping) of the fly strains used in artificial fly-breeding and share these data in research publications as well as when sharing cultures. In addition, fast-evolving nuclear markers should be used to reconstruct the recent invasion history of the species. |
Patrocinador/es: | This work was funded by the H2020 Research and Innovation Staff Exchange Programme of the European Commission (RISE), project 645636: ‘Insect-plant relationships: insights into biodiversity and new applications’ (FlyHigh), and the grants R-154-000-B46–114 and R-154-000-A94–592 to RM, as well as a grant of the Swiss Federal Office for Agriculture to CS (‘Efficiency and sustainability of the production and feeding of insect based feedstuff to fish and poultry’). |
URI: | http://hdl.handle.net/10045/107137 |
ISSN: | 1471-2148 |
DOI: | 10.1186/s12862-020-01627-2 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1186/s12862-020-01627-2 |
Aparece en las colecciones: | INV - BIONOMIA - Artículos Científicos / Scientific Papers Investigaciones financiadas por la UE |
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