A role for DJ-1 against oxidative stress in the mammalian retina
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Título: | A role for DJ-1 against oxidative stress in the mammalian retina |
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Autor/es: | Martín-Nieto, José | Uribe, Mary Luz | Esteve Rudd, Julián | Herrero Ezquerro, María Trinidad | Campello Blasco, Laura |
Grupo/s de investigación o GITE: | Genética Humana y de Mamíferos (GHM) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Palabras clave: | Parkinson disease | DJ-1 | Retina | Gene expression | Oxidative stress | Rotenone |
Área/s de conocimiento: | Genética |
Fecha de publicación: | 24-ago-2019 |
Editor: | Elsevier |
Cita bibliográfica: | Neuroscience Letters. 2019, 708: 134361. doi:10.1016/j.neulet.2019.134361 |
Resumen: | We have previously reported the expression of Parkinson disease-associated genes encoding α-synuclein, parkin and UCH-L1 in the retina across mammals. DJ-1, or parkinsonism-associated deglycase, is a redox-sensitive protein with putative roles in cellular protection against oxidative stress, among a variety of functions, acting through distinct pathways and mechanisms in a wide variety of tissues. Its function in counteracting oxidative stress in the retina, as it occurs in Parkinson and other human neurodegenerative diseases, is, however, poorly understood. In the present study, we address the expression of DJ-1 in the mammalian retina and its putative neuroprotective role in this tissue in a well-known model of parkinsonism, the rotenone-treated rat. As a result, we demonstrate that the DJ1 gene is expressed at both mRNA and protein levels in the neural retina and retinal pigment epithelium (RPE) of all mammalian species studied. We also present evidence that DJ-1 functions in the retina as a sensor of cellular redox homeostasis, which reacts to oxidative stress by increasing its intracellular levels and additionally becoming oxidized. Levels of α-synuclein also became upregulated, although parkin and UCH-L1 expression remained unchanged. It is inferred that DJ-1 likely exerts in the retina a potential neuroprotective role against oxidative stress, including α-synuclein oxidation and aggregation, which should be operative under both physiological and pathological conditions. |
Patrocinador/es: | This research was supported by grants from the Instituto de Salud Carlos III (PI09/1623 to JM-N, and PI10/02827 and PI13/01293 to MTH), cofinanced by the European Regional Development Fund (ERDF/FEDER), and from Fundación Séneca (19540/PI/14 to MTH). Additional funding was awarded by the Universidad de Alicante (UA) for use of technical research facilities (ref. UAUSTI18-16), diffusion of research results (ref. UADIF 18-52) and scientific productivity (ref. VIGROB-237). MLU, JE-R and LC were recipients of predoctoral contracts/fellowships from the UA. |
URI: | http://hdl.handle.net/10045/94474 |
ISSN: | 0304-3940 (Print) | 1872-7972 (Online) |
DOI: | 10.1016/j.neulet.2019.134361 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2019 Published by Elsevier B.V. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1016/j.neulet.2019.134361 |
Aparece en las colecciones: | INV - GHM - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2019_Martin-Nieto_etal_NeurosciLett_final.pdf | Versión final (acceso restringido) | 1,02 MB | Adobe PDF | Abrir Solicitar una copia |
2019_Martin-Nieto_etal_NeurosciLett_accepted.pdf | Accepted Manuscript (acceso abierto) | 588,25 kB | Adobe PDF | Abrir Vista previa |
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