Ethanol production from chitosan by the nematophagous fungus Pochonia chlamydosporia and the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/68998
Título: | Ethanol production from chitosan by the nematophagous fungus Pochonia chlamydosporia and the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana |
---|---|
Autor/es: | Aranda-Martínez, Almudena | Naranjo-Ortiz, Miguel Ángel | Abihssira García, Isabel Sofía | Zavala-González, Ernesto A. | Lopez-Llorca, Luis Vicente |
Grupo/s de investigación o GITE: | Fitopatología |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Palabras clave: | Biomass | Chitin | Chitosan | Chitosanases | Enzymatic hydrolysis | Bioethanol |
Área/s de conocimiento: | Botánica |
Fecha de publicación: | nov-2017 |
Editor: | Elsevier |
Cita bibliográfica: | Microbiological Research. 2017, 204: 30-39. doi:10.1016/j.micres.2017.07.009 |
Resumen: | Chitin is the second most abundant biopolymer after cellulose and virtually unexplored as raw material for bioethanol production. In this paper, we investigate chitosan, the deacetylated form of chitin which is the main component of shellfish waste, as substrate for bioethanol production by fungi. Fungal parasites of invertebrates such as the nematophagous Pochonia chlamydosporia (Pc) or the entomopathogens Beauveria bassiana (Bb) and Metarhizium anisopliae (Ma) are biocontrol agents of plant parasitic nematodes (eg. Meloidogyne spp.) or insect pests such as the red palm weevil (Rhynchophorus ferrugineus). These fungi degrade chitin-rich barriers for host penetration. We have therefore tested the chitin/chitosanolytic capabilities of Pc, Bb and Ma for generating reducing sugars using chitosan as only nutrient. Among the microorganisms used in this study, Pc is the best chitosan degrader, even under anaerobic conditions. These fungi have alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC) encoding genes in their genomes. We have therefore analyzed their ethanol production under anaerobic conditions using chitosan as raw material. P. chlamydosporia is the largest ethanol producer from chitosan. Our studies are a starting point to develop chitin-chitosan based biofuels. |
Patrocinador/es: | This work was supported by the Spanish Ministry of Economy and Competitiveness Grant AGL2015-66833-R and with a grant from the Generalitat Valenciana (Spain) to A. Aranda-Martinez (ACIF/2013/120). L.V. Lopez-Llorca was awarded a sabbatical grant (PR2015_00087) by the Spanish Ministry of Education, Culture and Sport. This work was filed for three patents (P201530552, P201530894 and P201530895) by L.V. Lopez-Llorca, M. A. Naranjo Ortiz and A. Aranda-Martinez, as inventors. |
URI: | http://hdl.handle.net/10045/68998 |
ISSN: | 0944-5013 (Print) | 1618-0623 (Online) |
DOI: | 10.1016/j.micres.2017.07.009 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2017 Elsevier GmbH |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.micres.2017.07.009 |
Aparece en las colecciones: | INV - Fitopatología - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
2017_Aranda_etal_MicrobiolRes_final.pdf | Versión final (acceso restringido) | 1,4 MB | Adobe PDF | Abrir Solicitar una copia |
Todos los documentos en RUA están protegidos por derechos de autor. Algunos derechos reservados.