Cell wall composition plays a key role on sensitivity of filamentous fungi to chitosan

Please use this identifier to cite or link to this item: http://hdl.handle.net/10045/62032
Información del item - Informació de l'item - Item information
Title: Cell wall composition plays a key role on sensitivity of filamentous fungi to chitosan
Authors: Aranda-Martínez, Almudena | Lopez-Moya, Federico | Lopez-Llorca, Luis Vicente
Research Group/s: Fitopatología
Center, Department or Service: Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef"
Keywords: Neurospora crassa | Pochonia chlamydosporia | Caspofungin | Chitosan | Antifungal
Knowledge Area: Botánica
Issue Date: Oct-2016
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
Citation: Journal of Basic Microbiology. 2016, 56(10): 1059-1070. doi:10.1002/jobm.201500775
Abstract: Chitosan antifungal activity has been reported for both filamentous fungi and yeast. Previous studies have shown fungal plasma membrane as main chitosan target. However, the role of the fungal cell wall (CW) in their response to chitosan is unknown. We show that cell wall regeneration in Neurospora crassa (chitosan sensitive) protoplasts protects them from chitosan damage. Caspofungin, a β-1,3-glucan synthase inhibitor, showed a synergistic antifungal effect with chitosan for N. crassa but not for Pochonia chlamydosporia, a biocontrol fungus resistant to chitosan. Chitosan significantly repressed N. crassa genes involved in β-1,3-glucan synthesis (fks) and elongation (gel-1) but the chitin synthase gene (chs-1) did not present changes in its expression. N. crassa cell wall deletion strains related to β-1,3-glucan elongation (Δgel-1 and Δgel-2) were more sensitive to chitosan than wild type (wt). On the contrary, chitin synthase deletion strain (Δchs-1) showed the same sensitivity to chitosan than wt. The mycelium of P. chlamydosporia showed a higher (ca. twofold) β-1,3-glucan/chitin ratio than that of N. crassa. Taken together, our results indicate that cell wall composition plays an important role on ­sensitivity of filamentous fungi to chitosan.
Sponsor: This research was funded by the Spanish Ministry of Economy and Competitiveness Grant AGL2015-66833-R and with a grant from the Generalitat Valenciana to A. Aranda-Martinez (ACIF/2013/120).
URI: http://hdl.handle.net/10045/62032
ISSN: 0233-111X (Print) | 1521-4028 (Online)
DOI: 10.1002/jobm.201500775
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2016 WILEY-VCH Verlag GmbH & Co.KGaA,Weinheim
Peer Review: si
Publisher version: http://dx.doi.org/10.1002/jobm.201500775
Appears in Collections:INV - Fitopatología - Artículos de Revistas

Files in This Item:
Files in This Item:
File Description SizeFormat 
Thumbnail2016_Aranda_etal_JBasicMicrobiol_final.pdfVersión final (acceso restringido)1,11 MBAdobe PDFOpen    Request a copy

Items in RUA are protected by copyright, with all rights reserved, unless otherwise indicated.