Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts
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Título: | Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts |
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Autor/es: | Barbosa, Oveimar | Ortiz, Claudia | Berenguer-Murcia, Ángel | Torres, Rodrigo | Rodrigues, Rafael C. | Fernández Lafuente, Roberto |
Grupo/s de investigación o GITE: | Materiales Carbonosos y Medio Ambiente |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Inorgánica | Universidad de Alicante. Instituto Universitario de Materiales |
Palabras clave: | Controlled immobilization | Enzyme stabilization | Multimeric enzymes | Chimeric proteins | Covalent attachment | Ionic exchange | IMAC |
Área/s de conocimiento: | Química Inorgánica |
Fecha de publicación: | 2015 |
Editor: | Elsevier |
Cita bibliográfica: | Biotechnology Advances. 2015, 33(5): 435-456. doi:10.1016/j.biotechadv.2015.03.006 |
Resumen: | In this review, we detail the efforts performed to couple the purification and the immobilization of industrial enzymes in a single step. The use of antibodies, the development of specific domains with affinity for some specific supports will be revised. Moreover, we will discuss the use of domains that increase the affinity for standard matrices (ionic exchangers, silicates). We will show how the control of the immobilization conditions may convert some unspecific supports in largely specific ones. The development of tailor-made heterofunctional supports as a tool to immobilize–stabilize–purify some proteins will be discussed in deep, using low concentration of adsorbent groups and a dense layer of groups able to give an intense multipoint covalent attachment. The final coupling of mutagenesis and tailor made supports will be the last part of the review. |
Patrocinador/es: | This work has been supported by grant CTQ2013-41507-R from Spanish MINECO, grant no.1102-489-25428 from COLCIENCIAS and Universidad Industrial de Santander (VIE-UIS Research Program) (Colombia) and CNPq grant 403505/2013-5 (Brazil). A. Berenguer-Murcia thanks the Spanish MINECO for a Ramon y Cajal fellowship (RyC-2009-03813). |
URI: | http://hdl.handle.net/10045/57890 |
ISSN: | 0734-9750 (Print) | 1873-1899 (Online) |
DOI: | 10.1016/j.biotechadv.2015.03.006 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2015 Elsevier Inc. |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.biotechadv.2015.03.006 |
Aparece en las colecciones: | INV - MCMA - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2015_Barbosa_etal_BiotechAdv_final.pdf | Versión final (acceso restringido) | 3,12 MB | Adobe PDF | Abrir Solicitar una copia |
2015_Barbosa_etal_BiotechAdv_preprint.pdf | Preprint (acceso abierto) | 407,11 kB | Adobe PDF | Abrir Vista previa |
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