A metabolomic approach to study the rhizodeposition in the tritrophic interaction: tomato, Pochonia chlamydosporia and Meloidogyne javanica
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http://hdl.handle.net/10045/37816
Títol: | A metabolomic approach to study the rhizodeposition in the tritrophic interaction: tomato, Pochonia chlamydosporia and Meloidogyne javanica |
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Autors: | Escudero Benito, Nuria | Marhuenda Egea, Frutos Carlos | Ibanco-Cañete, Ricardo | Zavala-González, Ernesto A. | Lopez-Llorca, Luis Vicente |
Grups d'investigació o GITE: | Fitopatología | Grupo de Fotoquímica y Electroquímica de Semiconductores (GFES) |
Centre, Departament o Servei: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Departamento de Agroquímica y Bioquímica | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Paraules clau: | Root exudates | Fluorescence spectroscopy | NMR | PARAFAC | HPLC–MS | Nematophagous fungus | Root-knot nematodes |
Àrees de coneixement: | Botánica | Bioquímica y Biología Molecular |
Data de publicació: | de febrer-2014 |
Editor: | Springer Science+Business Media New York |
Citació bibliogràfica: | Metabolomics. 2014, Febr. doi:10.1007/s11306-014-0632-3 |
Resum: | A combined chemometrics-metabolomics approach [excitation–emission matrix (EEM) fluorescence spectroscopy, nuclear magnetic resonance (NMR) and high performance liquid chromatography–mass spectrometry (HPLC–MS)] was used to analyse the rhizodeposition of the tritrophic system: tomato, the plant-parasitic nematode Meloidogyne javanica and the nematode-egg parasitic fungus Pochonia chlamydosporia. Exudates from M. javanica roots were sampled at root penetration (early) and gall development (late). EMM indicated that late root exudates from M. javanica treatments contained more aromatic amino acid compounds than the rest (control, P. chlamydosporia or P. chlamydosporia and M. javanica). 1H NMR showed that organic acids (acetate, lactate, malate, succinate and formic acid) and one unassigned aromatic compound (peak no. 22) were the most relevant metabolites in root exudates. Robust principal component analysis (PCA) grouped early exudates for nematode (PC1) or fungus presence (PC3). PCA found (PC1, 73.31 %) increased acetate and reduced lactate and an unassigned peak no. 22 characteristic of M. javanica root exudates resulting from nematode invasion and feeding. An increase of peak no. 22 (PC3, 4.82 %) characteristic of P. chlamydosporia exudates could be a plant “primer” defence. In late ones in PC3 (8.73 %) the presence of the nematode grouped the samples. HPLC–MS determined rhizosphere fingerprints of 16 (early) and 25 (late exudates) m/z signals, respectively. Late signals were exclusive from M. javanica exudates confirming EEM and 1H NMR results. A 235 m/z signal reduced in M. javanica root exudates (early and late) could be a repressed plant defense. This metabolomic approach and other rhizosphere -omics studies could help to improve plant growth and reduce nematode damage sustainably. |
Patrocinadors: | This research was funded by the Spanish Ministry of Science and Innovation Grants AGL 2008-00716/AGR, AGL 2011-29297 and with a grant from the University of Alicante to N. Escudero (UAFPU2011). |
URI: | http://hdl.handle.net/10045/37816 |
ISSN: | 1573-3882 (Print) | 1573-3890 (Online) |
DOI: | 10.1007/s11306-014-0632-3 |
Idioma: | eng |
Tipus: | info:eu-repo/semantics/article |
Drets: | The final publication is available at Springer via http://dx.doi.org/10.1007/s11306-014-0632-3 |
Revisió científica: | si |
Versió de l'editor: | http://dx.doi.org/10.1007/s11306-014-0632-3 |
Apareix a la col·lecció: | INV - GFES - Artículos de Revistas INV - Fitopatología - Artículos de Revistas |
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2014_Escudero_etal_Metabolomics_final.pdf | Versión final (acceso restringido) | 3,45 MB | Adobe PDF | Obrir Sol·licitar una còpia |
2014_Escudero_etal_Metabolomics.pdf | Versión revisada (acceso abierto) | 713,65 kB | Adobe PDF | Obrir Vista prèvia |
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