Computer vision profiling of neurite outgrowth dynamics reveals spatiotemporal modularity of Rho GTPase signaling
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Título: | Computer vision profiling of neurite outgrowth dynamics reveals spatiotemporal modularity of Rho GTPase signaling |
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Autor/es: | Fusco, Ludovico | Lefort, Riwal | Smith, Kevin | Benmansour, Fethallah | González, Germán | Barillari, Caterina | Rinn, Bernd | Fleuret, Francois | Fua, Pascal | Pertz, Olivier |
Grupo/s de investigación o GITE: | Robótica y Visión Tridimensional (RoViT) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencia de la Computación e Inteligencia Artificial |
Palabras clave: | Computer vision | Neuron profiling | Rho guanosine triphosphatases |
Fecha de publicación: | 4-ene-2016 |
Editor: | The Rockefeller University Press |
Cita bibliográfica: | Journal of Cell Biology. 2016, 212(1): 91-111. https://doi.org/10.1083/jcb.201506018 |
Resumen: | Rho guanosine triphosphatases (GTPases) control the cytoskeletal dynamics that power neurite outgrowth. This process consists of dynamic neurite initiation, elongation, retraction, and branching cycles that are likely to be regulated by specific spatiotemporal signaling networks, which cannot be resolved with static, steady-state assays. We present NeuriteTracker, a computer-vision approach to automatically segment and track neuronal morphodynamics in time-lapse datasets. Feature extraction then quantifies dynamic neurite outgrowth phenotypes. We identify a set of stereotypic neurite outgrowth morphodynamic behaviors in a cultured neuronal cell system. Systematic RNA interference perturbation of a Rho GTPase interactome consisting of 219 proteins reveals a limited set of morphodynamic phenotypes. As proof of concept, we show that loss of function of two distinct RhoA-specific GTPase-activating proteins (GAPs) leads to opposite neurite outgrowth phenotypes. Imaging of RhoA activation dynamics indicates that both GAPs regulate different spatiotemporal Rho GTPase pools, with distinct functions. Our results provide a starting point to dissect spatiotemporal Rho GTPase signaling networks that regulate neurite outgrowth. |
Patrocinador/es: | This work was supported by a Swiss National Science Foundation Sinergia grant to O. Pertz, P. Fua, and F. Fleuret and by Swiss National Science Foundation and International Foundation for Research in Paraplegia grants to O. Pertz. |
URI: | http://hdl.handle.net/10045/138558 |
ISSN: | 0021-9525 (Print) | 1540-8140 (Online) |
DOI: | 10.1083/jcb.201506018 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2016 Fusco et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1083/jcb.201506018 |
Aparece en las colecciones: | INV - RoViT - Artículos de Revistas |
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