Variable Intrinsic Expression of Immunoregulatory Biomarkers in Breast Cancer Cell Lines, Mammospheres, and Co-Cultures
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Título: | Variable Intrinsic Expression of Immunoregulatory Biomarkers in Breast Cancer Cell Lines, Mammospheres, and Co-Cultures |
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Autor/es: | Montoyo-Pujol, Yoel G. | García-Escolano, Marta | Ponce, Jose J. | Delgado-Garcia, Silvia | Martin, Tina A. | Ballester, Hortensia | Castellon-Molla, Elena | Martínez-Peinado, Pascual | Pascual-García, Sandra | Sempere Ortells, José Miguel | Peiró, Gloria |
Grupo/s de investigación o GITE: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Biotecnología |
Palabras clave: | Breast carcinoma | Immune checkpoints | mRNA expression | Cell lines | Mammospheres | Co-culture |
Fecha de publicación: | 24-feb-2023 |
Editor: | MDPI |
Cita bibliográfica: | Montoyo-Pujol YG, García-Escolano M, Ponce JJ, Delgado-García S, Martín TA, Ballester H, Castellón-Molla E, Martínez-Peinado P, Pascual-García S, Sempere-Ortells JM, Peiró G. Variable Intrinsic Expression of Immunoregulatory Biomarkers in Breast Cancer Cell Lines, Mammospheres, and Co-Cultures. International Journal of Molecular Sciences. 2023; 24(5):4478. https://doi.org/10.3390/ijms24054478 |
Resumen: | Advances in immunotherapy have increased interest in knowing the role of the immune system in breast cancer (BC) pathogenesis. Therefore, immune checkpoints (IC) and other pathways related to immune regulation, such as JAK2 and FoXO1, have emerged as potential targets for BC treatment. However, their intrinsic gene expression in vitro has not been extensively studied in this neoplasia. Thus, we evaluated the mRNA expression of tumor-cell-intrinsic CTLA-4, PDCD1 (PD1), CD274 (PD-L1), PDCD1LG2 (PD-L2), CD276 (B7-H3), JAK2, and FoXO1 in different BC cell lines, derived mammospheres, and co-cultures with peripheral blood mononuclear cells (PBMCs) by real-time quantitative polymerase chain reaction (qRT-PCR). Our results showed that intrinsic CTLA-4, CD274 (PD-L1), and PDCD1LG2 (PD-L2) were highly expressed in triple-negative cell lines, while CD276 was predominantly overexpressed in luminal cell lines. In contrast, JAK2 and FoXO1 were under-expressed. Moreover, high levels of CTLA-4, PDCD1 (PD1), CD274 (PD-L1), PDCD1LG2 (PD-L2), and JAK2 were found after mammosphere formation. Finally, the interaction between BC cell lines and peripheral blood mononuclear cells (PBMCs) stimulates the intrinsic expression of CTLA-4, PCDC1 (PD1), CD274 (PD-L1), and PDCD1LG2 (PD-L2). In conclusion, the intrinsic expression of immunoregulatory genes seems very dynamic, depending on BC phenotype, culture conditions, and tumor-immune cell interactions. |
Patrocinador/es: | This research was funded by the Alicante Institute for Health and Biomedical Research (ISABIAL) (NR-180202; NR-19-292; 2020-0324; 2021-0389; 2021-0453). |
URI: | http://hdl.handle.net/10045/132590 |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms24054478 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/ijms24054478 |
Aparece en las colecciones: | INV - Grupo de Inmunología - Artículos de Revistas |
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