Ultrasensitive determination of β-conglutin food allergen by means an aptamer assay based on inductively coupled plasma mass spectrometry detection
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Título: | Ultrasensitive determination of β-conglutin food allergen by means an aptamer assay based on inductively coupled plasma mass spectrometry detection |
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Autor/es: | Torregrosa Carretero, Daniel | Jauset-Rubio, Miriam | Serrano, Raquel | Svobodová, Marketa | Grindlay, Guillermo | O'Sullivan, Ciara K. | Mora, Juan |
Grupo/s de investigación o GITE: | Espectrometría Atómica Analítica (GEAA) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología |
Palabras clave: | β-conglutin | Aptamer assay | Nanoparticles | Single particle | Inductively coupled plasma mass spectrometry |
Fecha de publicación: | 3-mar-2023 |
Editor: | Elsevier |
Cita bibliográfica: | Analytica Chimica Acta. 2023, 1252: 341042. https://doi.org/10.1016/j.aca.2023.341042 |
Resumen: | The overall objective of this work is the evaluation of different competitive aptamer assays based on inductively coupled plasma mass spectrometry (ICP-MS) detection for the determination of β-conglutin (food protein allergen from lupin) in flour samples. To this end, two competitive aptamer assay schemes were developed using either thiolated aptamers chemisorbed onto gold nanoparticles (AuNPs) or biotinylated aptamers linked to streptavidin-AuNPs. The influence of ICP-MS detection mode (i.e., conventional vs single particle) on assay performance was explored. In the case of the thiolated aptamer, the limit of detection (LoD) obtained using the single particle mode was improved 2-fold as compared to the LoD provided by the conventional mode. With regards to the biotinylated aptamer, the use of the conventional mode provided a 5-fold improvement of LoD as compared to that obtained for the single particle one. Using the optimized conditions, the best LoD of 2 pM was obtained with the biotinylated aptamer operating with conventional ICP-MS detection. When compared to previous reports using the same aptamer in a competitive assay, the developed method significantly improved the LoD by at least an order of magnitude. Different flour samples containing lupin were successfully analyzed according to European Conformity guidelines for the analysis of food contaminants. |
Patrocinador/es: | The authors would like to thank the Generalitat Valenciana (Project GV/2014/138) and the Vice-Presidency for Research and Knowledge Transfer of the University of Alicante for the financial support of this work (Projects GRE12-19 and VIGROB-050). D. Torregrosa thanks the Spanish Ministerio de Ciencia, Innovación y Universidades for the given fellowship FPU17/02853. |
URI: | http://hdl.handle.net/10045/132479 |
ISSN: | 0003-2670 (Print) | 1873-4324 (Online) |
DOI: | 10.1016/j.aca.2023.341042 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1016/j.aca.2023.341042 |
Aparece en las colecciones: | INV - GEAA - Artículos de Revistas |
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Torregrosa_etal_2023_AnalChimActa.pdf | 2,55 MB | Adobe PDF | Abrir Vista previa | |
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