A Resistome Roadmap: From the Human Body to Pristine Environments
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/123547
Título: | A Resistome Roadmap: From the Human Body to Pristine Environments |
---|---|
Autor/es: | Maestre-Carballa, Lucia | Navarro-López, Vicente | Martinez-Garcia, Manuel |
Grupo/s de investigación o GITE: | Ecología Microbiana Molecular |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Antibiotic resistance genes | Human microbiome project | Pristine environments | Antibiotic resistance | Metagenomics |
Área/s de conocimiento: | Microbiología |
Fecha de publicación: | 11-may-2022 |
Editor: | Frontiers Media |
Cita bibliográfica: | Maestre-Carballa L, Navarro-López V and Martinez-Garcia M (2022) A Resistome Roadmap: From the Human Body to Pristine Environments. Front. Microbiol. 13:858831. doi: 10.3389/fmicb.2022.858831 |
Resumen: | A comprehensive characterization of the human body resistome [sets of antibiotic resistance genes (ARGs)] is yet to be done and paramount for addressing the antibiotic microbial resistance threat. Here, we study the resistome of 771 samples from five major body parts (skin, nares, vagina, gut, and oral cavity) of healthy subjects from the Human Microbiome Project (HMP) and addressed the potential dispersion of ARGs in pristine environments. A total of 28,714 ARGs belonging to 235 different ARG types were found in the HMP proteome dataset (n = 9.1 × 107 proteins analyzed). Our study reveals a distinct resistome profile (ARG type and abundance) between body sites and high interindividual variability. Nares had the highest ARG load (≈5.4 genes/genome) followed by the oral cavity, whereas the gut showed one of the highest ARG richness (shared with nares) but the lowest abundance (≈1.3 genes/genome). The fluroquinolone resistance genes were the most abundant in the human body, followed by macrolide–lincosamide–streptogramin (MLS) or tetracycline. Most ARGs belonged to common bacterial commensals and multidrug resistance trait were predominant in the nares and vagina. Many ARGs detected here were considered as low risk for human health, whereas only a few of them, such as BlaZ, dfrA14, dfrA17, or tetM, were classified as high-risk ARG. Our data also provide hope, since the spread of common ARG from the human body to pristine environments (n = 271 samples; 77 Gb of sequencing data and 2.1 × 108 proteins analyzed) thus far remains very unlikely (only one case found in an autochthonous bacterium from a pristine environment). These findings broaden our understanding of ARG in the context of the human microbiome and the One-Health Initiative of WHO uniting human host–microbes and environments as a whole. |
Patrocinador/es: | This project was supported by the Office of the Vice President for Research, Development, and Innovation (University of Alicante) with a grant from the Program “Predoctoral Training in Collaboration with Companies” (ref. UAIND18-05). Funds were also provided by the Hospital Elche Crevillente Salud SL (ref. HOSPITALECLHE1-18Y). |
URI: | http://hdl.handle.net/10045/123547 |
ISSN: | 1664-302X |
DOI: | 10.3389/fmicb.2022.858831 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2022 Maestre-Carballa, Navarro-López and Martinez-Garcia. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3389/fmicb.2022.858831 |
Aparece en las colecciones: | INV - EMM - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Maestre-Carballa_etal_2022_FrontMicrobiol.pdf | 1,45 MB | Adobe PDF | Abrir Vista previa | |
Este ítem está licenciado bajo Licencia Creative Commons