Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies
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Título: | Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies |
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Autor/es: | Rodrigues, Rafael C. | Berenguer-Murcia, Ángel | Carballares, Diego | Morellon-Sterling, Roberto | Fernández Lafuente, Roberto |
Grupo/s de investigación o GITE: | Materiales Carbonosos y Medio Ambiente |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Inorgánica | Universidad de Alicante. Instituto Universitario de Materiales |
Palabras clave: | Enzyme stabilization | Enzyme-support interactions | Lipase interfacial activation | Multipoint covalent immobilization | Multimeric enzymes | Glutaraldehyde | Glyoxyl | Epoxides | Vinyl sulfone |
Área/s de conocimiento: | Química Inorgánica |
Fecha de publicación: | 15-nov-2021 |
Editor: | Elsevier |
Cita bibliográfica: | Biotechnology Advances. 2021, 52: 107821. https://doi.org/10.1016/j.biotechadv.2021.107821 |
Resumen: | The use of enzymes in industrial processes requires the improvement of their features in many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse of these water-soluble catalysts, is one of the tools that researchers may utilize to improve many of their properties. This review is focused on how enzyme immobilization may improve enzyme stability. Starting from the stabilization effects that an enzyme may experience by the mere fact of being inside a solid particle, we detail other possibilities to stabilize enzymes: generation of favorable enzyme environments, prevention of enzyme subunit dissociation in multimeric enzymes, generation of more stable enzyme conformations, or enzyme rigidification via multipoint covalent attachment. In this last point, we will discuss the features of an “ideal” immobilization protocol to maximize the intensity of the enzyme-support interactions. The most interesting active groups in the support (glutaraldehyde, epoxide, glyoxyl and vinyl sulfone) will be also presented, discussing their main properties and uses. Some instances in which the number of enzyme-support bonds is not directly related to a higher stabilization will be also presented. Finally, the possibility of coupling site-directed mutagenesis or chemical modification to get a more intense multipoint covalent immobilization will be discussed. |
Patrocinador/es: | We gratefully recognize the financial support from Ministerio de Ciencia e Innovación-Spanish Government (project number CTQ2017-86170-R) and CSIC for the project AEP045. Diego Carballares thanks a FPI fellowship to Ministerio de Ciencia e Innovación, Roberto Morellon-Sterling recognizes a FPU to Ministerio de educación. |
URI: | http://hdl.handle.net/10045/118331 |
ISSN: | 0734-9750 (Print) | 1873-1899 (Online) |
DOI: | 10.1016/j.biotechadv.2021.107821 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1016/j.biotechadv.2021.107821 |
Aparece en las colecciones: | INV - MCMA - Artículos de Revistas |
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Archivo | Descripción | Tamaño | Formato | |
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Rodrigues_etal_2021_BiotechAdv.pdf | 7,45 MB | Adobe PDF | Abrir Vista previa | |
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