Metagenomics Unveils Posidonia oceanica “Banquettes” as a Potential Source of Novel Bioactive Compounds and Carbohydrate Active Enzymes (CAZymes)
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Título: | Metagenomics Unveils Posidonia oceanica “Banquettes” as a Potential Source of Novel Bioactive Compounds and Carbohydrate Active Enzymes (CAZymes) |
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Autor/es: | Rubio-Portillo, Esther | Martín Cuadrado, Ana Belén | Ramos-Esplá, Alfonso A. | Anton, Josefa |
Grupo/s de investigación o GITE: | Ecología Microbiana Molecular | Biología Marina |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada |
Palabras clave: | BGC | CAZyme | Myxococcota | NRPS | Posidonia oceanica | Banquettes | Bioactive compound | Pseudoalteromonas |
Área/s de conocimiento: | Microbiología | Zoología |
Fecha de publicación: | 14-sep-2021 |
Editor: | American Society for Microbiology |
Cita bibliográfica: | Rubio-Portillo E, Martin-Cuadrado A-B, Ramos-Esplá AÁ, Antón J. 2021. Metagenomics unveils Posidonia oceanica “banquettes” as a potential source of novel bioactive compounds and carbohydrate active enzymes (CAZymes). mSystems 6:e00866-21. https://doi.org/10.1128/mSystems.00866-21 |
Resumen: | Posidonia oceanica is a long-living and very slow-growing marine seagrass endemic to the Mediterranean Sea. It produces large amounts of leaf material and rhizomes, which can reach the shore and build important banks known as “banquettes.” In recent years, interest in the potential uses of these P. oceanica banquettes has increased, and it was demonstrated that biomass extracts showed antioxidant, antifungal, and antiviral activities. The discovery of new compounds through the culture of microorganisms is limited, and to overcome this limitation, we performed a metagenomic study to investigate the microbial community associated with P. oceanica banquettes. Our results showed that the microbial community associated with P. oceanica banquettes was dominated by Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes, and Cyanobacteria. Pseudoalteromonas was the dominant genus, followed by Alteromonas, Labrenzia, and Aquimarina. The metagenome reads were binned and assembled into 23 nearly complete metagenome-assembled genomes (MAGs), which belonged to new families of Cyanobacteria, Myxococcota, and Granulosicoccaceae and also to the novel genus recently described as Gammaproteobacteria family UBA10353. A comparative analysis with 60 published metagenomes from different environments, including seawater, marine biofilms, soils, corals, sponges, and hydrothermal vents, indicated that banquettes have numbers of natural products and carbohydrate active enzymes (CAZymes) similar to those found for soils and were only surpassed by marine biofilms. New proteins assigned to cellulosome modules and lignocellulose-degrading enzymes were also found. These results unveiled the diverse microbial composition of P. oceanica banquettes and determined that banquettes are a potential source of bioactive compounds and novel enzymes. |
Patrocinador/es: | This work was carried out within the project Metafluidics, funded by the European Union's framework program Horizon 2020 (grant no. LEIT‐BIO‐2015‐685474 to J.A.). |
URI: | http://hdl.handle.net/10045/117853 |
ISSN: | 2379-5077 |
DOI: | 10.1128/mSystems.00866-21 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 Rubio-Portillo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1128/mSystems.00866-21 |
Aparece en las colecciones: | INV - BM - Artículos Científicos / Scientific Papers INV - EMM - Artículos de Revistas Investigaciones financiadas por la UE |
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