Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
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Título: | Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal) |
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Autor/es: | Juan Vicedo, Jorge | Pavlov, Atanas | Ríos, Segundo | Casas, Jose L. |
Grupo/s de investigación o GITE: | Biodiversidad y Biotecnología aplicadas a la Biología de la Conservación |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias Ambientales y Recursos Naturales | Universidad de Alicante. Centro Iberoamericano de la Biodiversidad |
Palabras clave: | Bulb scales | Conservation | Endangered plants | Micropropagation | Narcissus | Plant growth regulators |
Área/s de conocimiento: | Botánica | Fisiología Vegetal |
Fecha de publicación: | 2021 |
Editor: | Polish Academy of Sciences | Jagiellonian University, Cracow |
Cita bibliográfica: | Acta Biologica Cracoviensia Series Botanica. 2021, 63(1): 55-61. https://doi.org/10.24425/abcsb.2020.131674 |
Resumen: | The genus Narcissus has several endemic, rare and/or threatened species in the Iberian Peninsula and North Africa. In vitro propagation is a useful tool for threatened plants conservation used in ex situ strategies. Thus, the aim of this work was to study the propagation in vitro of bulb scale explants of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula, treated with different PGR combinations. Initiation was achieved in half-strength Murashige and Skoog (MS) basal salts and vitamins, 10 g/L sucrose, 500 mg/L casein hydrolysate, 2 mg/L adenine, 10 mg/L glutathione and 5.5 g/L plant agar. In the multiplication phase, the highest bulblet proliferation was obtained in MS medium supplemented with 30 g/L sucrose and the combination of 10 μM 6-Benzylaminopurine (BAP) + 5 μM α-Naphthaleneacetic acid (NAA) in N. alcaracensis, N. eugeniae and N. hedraeanthus; 20 μM BAP + 5 μM NAA in N. jonquilla and N. yepesii. The highest rooting was obtained with 5 μM NAA + 1 μM Indole-3-butyric acid (IBA) for all species (>75%) and more than 80% of the produced bulblets were successfully acclimatized. |
Patrocinador/es: | The authors would like to acknowledge Universitat d’Alacant for the possibility of conducting this work by means of a grant included in the program “Becas y Contratos para la formación de Doctores del Vicerrectorado de Investigación, FPU2010” and specific funding for a research stay to start some of the cultures. Also, this work was partly supported by the R+D+I funding lines of the Spanish Ministry of Environment “Ministerio de Medio Ambiente y Medio Rural y Marino” through the project 126/SGTB/2007/4.1 ‘Caracterización, conservación y multiplicación de narcisos endémicos de Castilla-La Mancha (gen. Narcissus, Fam. Amaryllidaceae): revalorización de un recurso vegetal para el desarrollo sostenible de la Reserva de la Biosfera La Mancha Húmeda’. |
URI: | http://hdl.handle.net/10045/115298 |
ISSN: | 1898-0295 |
DOI: | 10.24425/abcsb.2020.131674 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © Polish Academy of Sciences and Jagiellonian University, Cracow 2021 |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.24425/abcsb.2020.131674 |
Aparece en las colecciones: | INV - BBaBC - Artículos de Revistas |
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Archivo | Descripción | Tamaño | Formato | |
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Juan-Vicedo_etal_2021_ABCbot.pdf | 185,38 kB | Adobe PDF | Abrir Vista previa | |
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