Use of Alcalase in the production of bioactive peptides: A review
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/110276
Título: | Use of Alcalase in the production of bioactive peptides: A review |
---|---|
Autor/es: | Tacias-Pascacio, Veymar G. | Morellon-Sterling, Roberto | Siar, El-Hocine | Tavano, Olga Luisa | Berenguer-Murcia, Ángel | Fernández Lafuente, Roberto |
Grupo/s de investigación o GITE: | Materiales Carbonosos y Medio Ambiente |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Inorgánica | Universidad de Alicante. Instituto Universitario de Materiales |
Palabras clave: | Protease immobilization | Protein hydrolysis | Bioactive peptides | Combienzymes | Enzyme selectivity | Enzyme specificity |
Área/s de conocimiento: | Química Inorgánica |
Fecha de publicación: | 15-dic-2020 |
Editor: | Elsevier |
Cita bibliográfica: | International Journal of Biological Macromolecules. 2020, 165(Part B): 2143-2196. https://doi.org/10.1016/j.ijbiomac.2020.10.060 |
Resumen: | This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization of Alcalase has also been reviewed, as immobilization of the enzyme may improve the final reaction design enabling the use of more drastic conditions and the reuse of the biocatalyst. That way, this review presents the production, via Alcalase hydrolysis of different proteins, of peptides with antioxidant, angiotensin I–converting enzyme inhibitory, metal binding, antidiabetic, anti-inflammatory and antimicrobial activities (among other bioactivities) and peptides that improve the functional, sensory and nutritional properties of foods. Alcalase has proved to be among the most efficient proteases for this goal, using different protein sources, being especially interesting the use of the protein residues from food industry as feedstock, as this also solves nature pollution problems. Very interestingly, the bioactivities of the protein hydrolysates further improved when Alcalase is used in a combined way with other proteases both in a sequential way or in a simultaneous hydrolysis (something that could be related to the concept of combi-enzymes), as the combination of proteases with different selectivities and specificities enable the production of a larger amount of peptides and of a smaller size. |
Patrocinador/es: | We gratefully recognize the support from the Ministerio de Ciencia e Innovación from Spanish Government (project number CTQ2017-86170-R). The FPU fellowship (Ministerio de Educacion) for Mr. Morellon–Sterling and the fellowship for Mr. Siar from the Algerian Ministry of Higher Education and Scientific Research are also thanked. Dr. Tacias-Pascacio thanks the financial support from “Programa para el Desarrollo Profesional Docente” (PRODEP) from Mexican Government. |
URI: | http://hdl.handle.net/10045/110276 |
ISSN: | 0141-8130 (Print) | 1879-0003 (Online) |
DOI: | 10.1016/j.ijbiomac.2020.10.060 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2020 Elsevier B.V. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1016/j.ijbiomac.2020.10.060 |
Aparece en las colecciones: | INV - MCMA - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Tacias-Pascacio_etal_2020_IntJBiolMacromol_final.pdf | Versión final (acceso restringido) | 2,29 MB | Adobe PDF | Abrir Solicitar una copia |
Tacias-Pascacio_etal_2020_IntJBiolMacromol_accepted.pdf | Accepted Manuscript (acceso abierto) | 6,79 MB | Adobe PDF | Abrir Vista previa |
Todos los documentos en RUA están protegidos por derechos de autor. Algunos derechos reservados.