Cytotoxicity of the ascidian Cystodytes dellechiajei against tumor cells and study of the involvement of associated microbiota in the production of cytotoxic compounds

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Title: Cytotoxicity of the ascidian Cystodytes dellechiajei against tumor cells and study of the involvement of associated microbiota in the production of cytotoxic compounds
Authors: Martinez-Garcia, Manuel | Díaz Valdés, Marta | Ramos-Esplá, Alfonso A. | Salvador, Nélida | López, Patricia | Larriba Tornel, Eduardo | Anton, Josefa
Research Group/s: Biología Marina
Center, Department or Service: Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. División de Microbiología | Instituto Biomar
Keywords: Ascidian | Cytotoxicity | Tumor | Cystodytes | Bacteria
Knowledge Area: Biología Marina | Microbiología | Zoología
Date Created: 18-Jun-2007
Issue Date: 19-Jul-2007
Publisher: Molecular Diversity Preservation International (MDPI)
Citation: MARTÍNEZ GARCÍA, Manuel, et al. “Cytotoxicity of the ascidian Cystodytes dellechiajei against tumor cells and study of the involvement of associated microbiota in the production of cytotoxic compounds”. Marine Drugs. Vol. 65, Issue 3 (Sept. 2007). ISSN 1660-3397, pp. 52-70
Abstract: Many cytotoxic compounds of therapeutic interest have been isolated from marine invertebrates, and some of them have been reported to be of microbial origin. Pyridoacridine alkaloids are the main compounds extracted from the ascidian Cystodytes dellechiajei. Here we describe the in vitro antiproliferative activity against different tumor cell lines of the ascidian extracts and provide some insights on the role of the microbial community associated with the tunicate in the production of these compounds. C. dellechiajei extracts showed remarkably high antiproliferative activity (IC50 ≤5 μg/mL) in human lung carcinoma A-549, colon adenocarcinoma H-116, pancreatic adenocarcinoma PSN-1 and breast carcinoma SKBR3 cell lines. Moreover, we found that the maximum activity was located in the tunic tissue of the colony, which harbours a microbial community. In order to ascertain the involvement of this community in the synthesis of the bioactive compounds different approachs that included culture and culture independent methods were carried out. We undertook a screening for antiproliferative activities of the bacterial isolates from the ascidian, as well as a comparative analysis of the cytotoxic activities and the microbial communities from two color morphs of the ascidian, green and blue. In addition, the changes of the antiproliferative activities and the composition of the microbial communities were studied from ascidians kept in aquaria and treated with antibiotics for one month. Our data obtained from the different experiments did not point out to bacteria as the source of the cytotoxic compounds, suggesting thus an ascidian origin.
Sponsor: This work was supported by grant BIO2000-005-P4-04 from the Spanish Ministry of Science and Education.
URI: http://hdl.handle.net/10045/9610
ISSN: 1660-3397
DOI: 10.3390/md503052
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2007 by MDPI (http://www.mdpi.org). Reproduction is permitted for noncommercial purposes.
Peer Review: si
Appears in Collections:INV - BM - Artículos Científicos / Scientific Papers
INV - EMM - Artículos de Revistas

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