Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan
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Título: | Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan |
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Autor/es: | Lopez-Moya, Federico | Escudero Benito, Nuria | Zavala-González, Ernesto A. | Esteve-Bruna, David | Blázquez, Miguel A. | Alabadí, David | Lopez-Llorca, Luis Vicente |
Grupo/s de investigación o GITE: | Fitopatología |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Palabras clave: | Chitosan | Auxin biosynthesis | WOX5 repression | Root development | Arabidopsis |
Área/s de conocimiento: | Botánica |
Fecha de publicación: | 1-dic-2017 |
Editor: | Springer Nature |
Cita bibliográfica: | Scientific Reports. 2017, 7: 16813. doi:10.1038/s41598-017-16874-5 |
Resumen: | Chitosan is a natural polymer with applications in agriculture, which causes plasma membrane permeabilisation and induction of intracellular reactive oxygen species (ROS) in plants. Chitosan has been mostly applied in the phylloplane to control plant diseases and to enhance plant defences, but has also been considered for controlling root pests. However, the effect of chitosan on roots is virtually unknown. In this work, we show that chitosan interfered with auxin homeostasis in Arabidopsis roots, promoting a 2–3 fold accumulation of indole acetic acid (IAA). We observed chitosan dose-dependent alterations of auxin synthesis, transport and signalling in Arabidopsis roots. As a consequence, high doses of chitosan reduce WOX5 expression in the root apical meristem and arrest root growth. Chitosan also propitiates accumulation of salicylic (SA) and jasmonic (JA) acids in Arabidopsis roots by induction of genes involved in their biosynthesis and signalling. In addition, high-dose chitosan irrigation of tomato and barley plants also arrests root development. Tomato root apices treated with chitosan showed isodiametric cells respect to rectangular cells in the controls. We found that chitosan causes strong alterations in root cell morphology. Our results highlight the importance of considering chitosan dose during agronomical applications to the rhizosphere. |
Patrocinador/es: | This work was supported by AGL 2015 66833-R Grant from the Spanish Ministry of Economy and Competitiveness Grant AGL 2015. |
URI: | http://hdl.handle.net/10045/71790 |
ISSN: | 2045-2322 |
DOI: | 10.1038/s41598-017-16874-5 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1038/s41598-017-16874-5 |
Aparece en las colecciones: | INV - Fitopatología - Artículos de Revistas |
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