Reprint of: l-Cysteine determination in embryo cell culture media using Co (II)-phthalocyanine modified disposable screen-printed electrodes
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10045/68569
Title: | Reprint of: l-Cysteine determination in embryo cell culture media using Co (II)-phthalocyanine modified disposable screen-printed electrodes |
---|---|
Authors: | Hernández Ibáñez, Naiara | Sanjuán, Ignacio | Montiel, Miguel A. | Foster, Christopher W. | Banks, Craig E. | Iniesta, Jesus |
Research Group/s: | Electroquímica Aplicada y Electrocatálisis |
Center, Department or Service: | Universidad de Alicante. Departamento de Química Física | Universidad de Alicante. Instituto Universitario de Electroquímica |
Keywords: | Sthiol-containing compounds | l-Cysteine | Cobalt phthalocyanine | Screen-printed electrodes | Embryo cell culture media | Oxidative stress |
Knowledge Area: | Química Física |
Issue Date: | 15-May-2017 |
Publisher: | Elsevier |
Citation: | Journal of Electroanalytical Chemistry. 2017, 793: 77-84. doi:10.1016/j.jelechem.2017.04.034 |
Abstract: | Thiol-containing compounds such as l-cysteine have been demonstrated to play an important role in metabolism and cellular growth, acting as powerful antioxidants. Consequently, their analytical determination in biological media has received a considerable amount of attention. In this work, an electrochemical sensor for the accurate electroanalytical determination of l-cysteine is proposed, based upon a Co(II)-phthalocyanine bulk modified disposable screen-printed graphite electrode (CoPc-SPE). Cyclic (CV) and Square Wave (SWV) voltammetry experiments have demonstrated an excellent electrocatalytic activity towards the electrochemical oxidation of l-cysteine using CoPc-SPEs within optimum neutral or basic pH. Moreover, the SWV response of l-cysteine is found to exhibit a linear range of 2.6–200 μM, with a low limit of detection of 4 μM (S/N = 3) and a sensitivity of 0.78 μA cm− 2 μM− 1. Coefficient of variation for reproducibility and repeatability of l-cysteine determination using the CoPc-SPE are 3 and 0.4%, respectively. The effect of inherent interferences such as naturally occurring amino acids, cystine and cysteic acid has been also evaluated. Finally, the applicability of the l-cysteine electrochemical sensor based upon CoPc-SPEs has been successfully demonstrated for the first time for the assessment of l-cysteine in a complex embryo cell culture medium. |
Sponsor: | J. Iniesta thanks the programme Salvador de Madariaga from the Ministerio de Economía y Competitividad, grant number PRX14/00363. This work has been also financially supported by the MICINN-FEDER (Spain) through the projects CTQ2013-48280-C3-3-R. |
URI: | http://hdl.handle.net/10045/68569 |
ISSN: | 1572-6657 (Print) | 1873-2569 (Online) |
DOI: | 10.1016/j.jelechem.2017.04.034 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2016 Published by Elsevier B.V. |
Peer Review: | si |
Publisher version: | http://dx.doi.org/10.1016/j.jelechem.2017.04.034 |
Appears in Collections: | INV - LEQA - Artículos de Revistas |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
2017_Hernandez_etal_JElectrChem_final.pdf | Versión final (acceso restringido) | 1,41 MB | Adobe PDF | Open Request a copy |
Items in RUA are protected by copyright, with all rights reserved, unless otherwise indicated.