Au-IDA microelectrodes modified with Au-doped graphene oxide for the simultaneous determination of uric acid and ascorbic acid in urine samples
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Título: | Au-IDA microelectrodes modified with Au-doped graphene oxide for the simultaneous determination of uric acid and ascorbic acid in urine samples |
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Autor/es: | Abellán-Llobregat, Alejandra | Vidal, Lorena | Rodríguez-Amaro, Rafael | Berenguer-Murcia, Ángel | Canals, Antonio | Morallon, Emilia |
Grupo/s de investigación o GITE: | Espectroscopía Atómica-Masas y Química Analítica en Condiciones Extremas | Materiales Carbonosos y Medio Ambiente | Electrocatálisis y Electroquímica de Polímeros |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología | Universidad de Alicante. Departamento de Química Física | Universidad de Alicante. Instituto Universitario de Materiales |
Palabras clave: | Electrochemical sensors | Graphene oxide | Gold nanoparticles | Uric acid | Ascorbic acid |
Área/s de conocimiento: | Nutrición y Bromatología | Química Inorgánica | Química Analítica | Química Física |
Fecha de publicación: | 10-feb-2017 |
Editor: | Elsevier |
Cita bibliográfica: | Electrochimica Acta. 2017, 227: 275-284. doi:10.1016/j.electacta.2016.12.132 |
Resumen: | An electrochemical sensor based on graphene oxide decorated with gold nanoparticles has been prepared for the simultaneous quantification of uric acid (UA) and ascorbic acid (AA) in urine samples. The gold interdigitated microelectrodes array (Au-IDA) has been modified using graphene oxide doped with gold nanoparticles (AuNPs-GO/Au-IDA), which was characterized by TEM, FE-SEM, XPS and cyclic voltammetry. Excellent results were obtained for the separate quantification of UA and AA by chronoamperometry. The electrochemical sensor exhibits limits of detection (LODs) of 1.4 μM and 0.62 μM for AA and UA, respectively, limits of quantification (LOQs) of 4.6 μM (AA) and 2.0 μM (UA), and the working ranges obtained were between 4.6 μM and 193 μM for AA and between 2 μM and 1.05 mM for UA. The repeatability was studied at 20 μM providing coefficients of variation of 16% for AA and 13% for UA. Moreover, UA does not interfere in the measurement of AA and viceversa (provided that the concentration of UA is equal to or higher than 450 μM in the latter case). For lower concentrations of UA, an easy and fast strategy to quantify both analytes is presented. The good electrocatalytic activity achieved with this material makes it useful for the quantification of AA and UA in biological fluids. Other analytes like glucose, dopamine and epinephrine have been investigated. The results allow us to conclude that they do not interfere in the quantification of AA and UA in PBS (0.25 M, pH 7.0). Human urine samples have been analyzed using the method proposed, contaning AA and UA concentration levels of (0.588 ± 0.002) mM and (1.43 ± 0.02) mM, respectively, which are in the concentration range of these analytes in urine samples for healthy people. |
Patrocinador/es: | This work is supported by the Generalitat Valenciana (Prometeo2013/038 and PROMETEOII/2014/010) and by the Ministerio de Economia y Competitividad (MAT201676595-R). A. Abellán-Llobregat also thanks the Generalitat Valenciana for her fellowship. |
URI: | http://hdl.handle.net/10045/61793 |
ISSN: | 0013-4686 (Print) | 1873-3859 (Online) |
DOI: | 10.1016/j.electacta.2016.12.132 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2016 Elsevier Ltd. |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.electacta.2016.12.132 |
Aparece en las colecciones: | INV - MCMA - Artículos de Revistas INV - SP-BG - Artículos de Revistas INV - GEPE - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2017_Abellan_etal_ElectActa_final.pdf | Versión final (acceso restringido) | 4,26 MB | Adobe PDF | Abrir Solicitar una copia |
2017_Abellan_etal_ElectActa_accepted.pdf | Accepted Manuscript (acceso abierto) | 968,27 kB | Adobe PDF | Abrir Vista previa |
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