Au-IDA microelectrodes modified with Au-doped graphene oxide for the simultaneous determination of uric acid and ascorbic acid in urine samples

Please use this identifier to cite or link to this item:
Información del item - Informació de l'item - Item information
Title: Au-IDA microelectrodes modified with Au-doped graphene oxide for the simultaneous determination of uric acid and ascorbic acid in urine samples
Authors: Abellán-Llobregat, Alejandra | Vidal, Lorena | Rodríguez-Amaro, Rafael | Berenguer-Murcia, Ángel | Canals, Antonio | Morallon, Emilia
Research Group/s: Espectroscopía Atómica-Masas y Química Analítica en Condiciones Extremas | Materiales Carbonosos y Medio Ambiente | Electrocatálisis y Electroquímica de Polímeros
Center, Department or Service: Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología | Universidad de Alicante. Departamento de Química Física | Universidad de Alicante. Instituto Universitario de Materiales
Keywords: Electrochemical sensors | Graphene oxide | Gold nanoparticles | Uric acid | Ascorbic acid
Knowledge Area: Nutrición y Bromatología | Química Inorgánica | Química Analítica | Química Física
Issue Date: 10-Feb-2017
Publisher: Elsevier
Citation: Electrochimica Acta. 2017, 227: 275-284. doi:10.1016/j.electacta.2016.12.132
Abstract: An electrochemical sensor based on graphene oxide decorated with gold nanoparticles has been prepared for the simultaneous quantification of uric acid (UA) and ascorbic acid (AA) in urine samples. The gold interdigitated microelectrodes array (Au-IDA) has been modified using graphene oxide doped with gold nanoparticles (AuNPs-GO/Au-IDA), which was characterized by TEM, FE-SEM, XPS and cyclic voltammetry. Excellent results were obtained for the separate quantification of UA and AA by chronoamperometry. The electrochemical sensor exhibits limits of detection (LODs) of 1.4 μM and 0.62 μM for AA and UA, respectively, limits of quantification (LOQs) of 4.6 μM (AA) and 2.0 μM (UA), and the working ranges obtained were between 4.6 μM and 193 μM for AA and between 2 μM and 1.05 mM for UA. The repeatability was studied at 20 μM providing coefficients of variation of 16% for AA and 13% for UA. Moreover, UA does not interfere in the measurement of AA and viceversa (provided that the concentration of UA is equal to or higher than 450 μM in the latter case). For lower concentrations of UA, an easy and fast strategy to quantify both analytes is presented. The good electrocatalytic activity achieved with this material makes it useful for the quantification of AA and UA in biological fluids. Other analytes like glucose, dopamine and epinephrine have been investigated. The results allow us to conclude that they do not interfere in the quantification of AA and UA in PBS (0.25 M, pH 7.0). Human urine samples have been analyzed using the method proposed, contaning AA and UA concentration levels of (0.588 ± 0.002) mM and (1.43 ± 0.02) mM, respectively, which are in the concentration range of these analytes in urine samples for healthy people.
Sponsor: This work is supported by the Generalitat Valenciana (Prometeo2013/038 and PROMETEOII/2014/010) and by the Ministerio de Economia y Competitividad (MAT201676595-R). A. Abellán-Llobregat also thanks the Generalitat Valenciana for her fellowship.
ISSN: 0013-4686 (Print) | 1873-3859 (Online)
DOI: 10.1016/j.electacta.2016.12.132
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2016 Elsevier Ltd.
Peer Review: si
Publisher version:
Appears in Collections:INV - MCMA - Artículos de Revistas
INV - SP-BG - Artículos de Revistas
INV - GEPE - Artículos de Revistas

Files in This Item:
Files in This Item:
File Description SizeFormat 
Thumbnail2017_Abellan_etal_ElectActa_final.pdfVersión final (acceso restringido)4,26 MBAdobe PDFOpen    Request a copy
Thumbnail2017_Abellan_etal_ElectActa_accepted.pdfAccepted Manuscript (acceso abierto)968,27 kBAdobe PDFOpen Preview

Items in RUA are protected by copyright, with all rights reserved, unless otherwise indicated.