Inherited Photoreceptor Degeneration Causes the Death of Melanopsin-Positive Retinal Ganglion Cells and Increases Their Coexpression of Brn3a
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Campo DC | Valor | Idioma |
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dc.contributor | Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) | es_ES |
dc.contributor.author | García Ayuso, Diego | - |
dc.contributor.author | Di Pierdomenico, Johnny | - |
dc.contributor.author | Esquiva, Gema | - |
dc.contributor.author | Nadal-Nicolás, Francisco Manuel | - |
dc.contributor.author | Pinilla Lozano, Isabel | - |
dc.contributor.author | Cuenca, Nicolás | - |
dc.contributor.author | Vidal Sanz, Manuel | - |
dc.contributor.author | Agudo Barriuso, Marta | - |
dc.contributor.author | Villegas Pérez, María Paz | - |
dc.contributor.other | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | es_ES |
dc.date.accessioned | 2016-09-15T09:58:03Z | - |
dc.date.available | 2016-09-15T09:58:03Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.citation | Investigative Ophthalmology & Visual Science. 2015, 56: 4592-4604. doi:10.1167/iovs.15-16808 | es_ES |
dc.identifier.issn | 0146-0404 (Print) | - |
dc.identifier.issn | 1552-5783 (Online) | - |
dc.identifier.uri | http://hdl.handle.net/10045/57937 | - |
dc.description.abstract | Purpose: To study the population of intrinsically photosensitive retinal ganglion cells (melanopsin-expressing RGCs, m+RGCs) in P23H-1 rats, a rat model of inherited photoreceptor degeneration. Methods: At postnatal (P) times P30, P365, and P540, retinas from P23H dystrophic rats (line 1, rapid degeneration; and line 3, slow degeneration) and Sprague Dawley (SD) rats (control) were dissected as whole-mounts and immunodetected for melanopsin and/or Brn3a. The dendritic arborization of m+RGCs and the numbers of Brn3a+RGCs and m+RGCs were quantified and their retinal distribution and coexpression analyzed. Results: In SD rats, aging did not affect the population of Brn3a+RGCs or m+RGCs or the percentage that showed coexpression (0.27%). Young P23H-1 rats had a significantly lower number of Brn3a+RGCs and showed a further decline with age. The population of m+RGCs in young P23H-1 rats was similar to that found in SD rats and decreased by 22.6% and 28.2% at P365 and P540, respectively, similarly to the decrease of the Brn3a+RGCs. At these ages the m+RGCs showed a decrease of their dendritic arborization parameters, which was similar in both the P23H-1 and P23H-3 lines. The percentage of coexpression of Brn3a was, however, already significantly higher at P30 (3.31%) and increased significantly with age (10.65% at P540). Conclusions: Inherited photoreceptor degeneration was followed by secondary loss of Brn3a+RGCs and m+RGCs. Surviving m+RGCs showed decreased dendritic arborization parameters and increased coexpression of Brn3a and melanopsin, phenotypic and molecular changes that may represent an effort to resist degeneration and/or preferential survival of m+RGCs capable of synthesizing Brn3a. | es |
dc.description.sponsorship | Supported by grants from the Spanish Ministry of Economy and Competitiveness: SAF-2012-38328; ISCIII-FEDER “Una manera de hacer Europa” PI13/00643, PI13/01266, and BFU2012-36845, RETICS: RD12/0034/0014, and RD12/0034/0010. | es |
dc.language | eng | es |
dc.publisher | Association for Research in Vision and Ophthalmology (ARVO) | es_ES |
dc.rights | © 2015 by Association for Research in Vision and Ophthalmology | es |
dc.subject | P23H | es |
dc.subject | Retinitis pigmentosa | es |
dc.subject | Melanopsin | es |
dc.subject | Brn3a | es |
dc.subject | Automated quantification | es |
dc.subject | Intrinsically photosensitive | es |
dc.subject | Photoreceptor degeneration | es |
dc.subject | Intrinsically photosensitive RGCs | es |
dc.subject.other | Biología Celular | es_ES |
dc.title | Inherited Photoreceptor Degeneration Causes the Death of Melanopsin-Positive Retinal Ganglion Cells and Increases Their Coexpression of Brn3a | es_ES |
dc.type | info:eu-repo/semantics/article | es |
dc.peerreviewed | si | es |
dc.identifier.doi | 10.1167/iovs.15-16808 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1167/iovs.15-16808 | es |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | es |
Aparece en las colecciones: | INV - NEUROVIS - Artículos de Revistas |
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