Importance of the Support Properties for Immobilization or Purification of Enzymes
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Título: | Importance of the Support Properties for Immobilization or Purification of Enzymes |
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Autor/es: | Santos, Jose Cleiton S. dos | Barbosa, Oveimar | Ortiz, Claudia | Berenguer-Murcia, Ángel | Rodrigues, Rafael C. | Fernández Lafuente, Roberto |
Grupo/s de investigación o GITE: | Materiales Carbonosos y Medio Ambiente |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Inorgánica | Universidad de Alicante. Instituto Universitario de Materiales |
Palabras clave: | Enzyme purification | Immobilization | Multipoint attachment | One-point attachment | Stabilization | Superficial density of reactive groups |
Área/s de conocimiento: | Química Inorgánica |
Fecha de publicación: | 17-ago-2015 |
Editor: | Wiley-VCH Verlag GmbH & Co. KGaA |
Cita bibliográfica: | ChemCatChem. 2015, 7(16): 2413-2432. doi:10.1002/cctc.201500310 |
Resumen: | Immobilization and purification of enzymes are usual requirements for their industrial use. Both purification and immobilization have a common factor: they use a solid activated support. Using a support for enzyme purification means having mild conditions for enzyme release and a selective enzyme–support interaction is interesting. When using a support for immobilization, however, enzyme desorption is a problem. The improvement of enzyme features through immobilization is a usual objective (e.g., stability, selectivity). Thus, a support designed for enzyme purification and a support designed for enzyme immobilization may differ significantly. In this review, we will focus our attention on the requirements of a support surface to produce the desired objectives. The ideal physical properties of the matrix, the properties of the introduced reactive groups, the best surface activation degree to reach the desired objective, and the properties of the reactive groups will be discussed. |
Patrocinador/es: | We gratefully recognize the support from the Spanish Government, CTQ2013-41507-R, Colciencia (Colombia) and CNPq (Brazil). The predoctoral fellowships for Mr dos Santos (CNPq, Brazil) are also recognized. Á. Berenguer-Murcia thanks the Spanish Ministerio de Ciencia e Innovaciûn for a Ramón y Cajal fellowship (RyC-2009-03813). |
URI: | http://hdl.handle.net/10045/57899 |
ISSN: | 1867-3880 (Print) | 1867-3899 (Online) |
DOI: | 10.1002/cctc.201500310 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1002/cctc.201500310 |
Aparece en las colecciones: | INV - MCMA - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2015_Santos_etal_ChemCatChem_final.pdf | Versión final (acceso restringido) | 3,66 MB | Adobe PDF | Abrir Solicitar una copia |
2015_Santos_etal_ChemCatChem_preprint.pdf | Preprint (acceso abierto) | 978,56 kB | Adobe PDF | Abrir Vista previa |
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