Assessment of oxidative stress markers and prostaglandins after chronic training of triathletes

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Título: Assessment of oxidative stress markers and prostaglandins after chronic training of triathletes
Autor/es: Medina Escudero, Sonia | Domínguez Perles, Raúl | Cejuela Anta, Roberto | Villaño Valencia, Débora | Martínez Sanz, José Miguel | Gil, Pilar | García Viguera, Cristina | Ferreres de Arce, Federico | Gil, José I. | Gil-Izquierdo, Ángel
Grupo/s de investigación o GITE: Grupo de Investigación Interdisciplinar en Docencia Universitaria (GIDU)
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Didáctica General y Didácticas Específicas
Palabras clave: Isoprostane | Oxidative stress | Prostaglandin | Thromboxane | Triathletes | Enzymatic hydrolysis
Área/s de conocimiento: Nutrición y Bromatología | Educación Física y Deportiva
Fecha de publicación: 21-jul-2012
Editor: Elsevier
Cita bibliográfica: MEDINA, Sonia, et al. “Assessment of oxidative stress markers and prostaglandins after chronic training of triathletes”. Prostaglandins & Other Lipid Mediators. In Press, Corrected Proof, Available online 21 July 2012. ISSN 0090-6980
Resumen: The present study established the pattern of isoprostanes (IsoPs) and prostaglandins metabolites (PGMs) in urine after triathlon training. Fifteen Caucasian triathletes – 5 women and 10 men – performed 793 and 1603 Objective Load Scales, respectively. The optimization of urine hydrolysis conditions, concerning to the type of buffer, the units of hydrolytic enzyme added, and the pH, allowed precise quantification of these metabolites by UPLC–MS/MS, avoiding the under-estimation of their concentrations that occurred in previous studies. Their rate of conjugation ranged between 36% and 100%. This implies significant importance since it supposes non-detection of some IsoPs and PGMs totally conjugated with glucuronic acid developed by other previous methodologies. Among the 13 compounds analyzed, this assay detected and characterized 4 IsoPs and 3 PGMs in the triathletes’ urine. The PGMs tetranor-PGEM and 11β-PGF2α and the IsoP 8-iso-PGF2α, showed lower concentrations after the training program, whereas the PGMs 6-keto-PGF1α increased (vascular PGI2 metabolite). In fact, their pattern in the triathletes’ urine indicated that their variation may have been related with the physical activity. Due to its high variation, 6-keto PGF1α stood out as a useful marker of the vasodilation and inhibition of the platelet aggregation of the PGI2 linked to the physical exercise. The data obtained provided a global picture of changes in lipid peroxidation and vascular events as a consequence of chronic exercise.
Patrocinador/es: The authors are grateful for the support of the National Funding Agencies through the Projects AGL2011-23690 (CICYT), CSD007-0063 (CONSOLIDER-INGENIO 2010 ‘Fun-C-Food’), and CSIC 201170E041 (Spanish Ministry of Economy and Competitiveness). They are also grateful to the Fundación Séneca – CARM ‘Group of Excellence in Research’ 04486/GERM/06 and the Ibero-American Programme for Science, Technology and Development (CYTED) – Action 112RT0460 CORNUCOPIA. SME was granted a pre-doctoral CSIC-JAE fellowship. RDP was appointed under a CSIC research contract.
URI: http://hdl.handle.net/10045/24670
ISSN: 0090-6980 (Print) | 1878-416X (Online)
DOI: 10.1016/j.prostaglandins.2012.07.002
Idioma: eng
Tipo: info:eu-repo/semantics/article
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1016/j.prostaglandins.2012.07.002
Aparece en las colecciones:INV - GIDU - Artículos de Revistas
INV - ALINUT - Artículos de Revistas
INV - RIPEFAP - Artículos de Revistas

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