Retinal degeneration in two lines of transgenic S334ter rats
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Título: | Retinal degeneration in two lines of transgenic S334ter rats |
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Autor/es: | Martínez Navarrete, Gema Concepción | Seiler, M.J. | Aramant, R.B. | Fernández-Sánchez, Laura | Pinilla Lozano, Isabel | Cuenca, Nicolás |
Grupo/s de investigación o GITE: | Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Retinal degeneration | Rodent | Remodeling | Apoptosis | Protein kinase C | Calbindin | Recoverin | Parvalbumin | Transducin |
Área/s de conocimiento: | Oftalmología | Fisiología |
Fecha de publicación: | 11-dic-2010 |
Editor: | Elsevier |
Cita bibliográfica: | MARTÍNEZ NAVARRETE, Gema C., et al. "Retinal degeneration in two lines of transgenic S334ter rats". Experimental Eye Research. Vol. 92, No. 3 (March 2011). ISSN 0014-4835, pp. 227-237 |
Resumen: | Aim of this study was to examine synaptic connectivity changes in the retina and the location and rate of apoptosis in transgenic S334ter line-3 and line-5 rats with photoreceptor degeneration. Heterozygous S334ter-line-3 and line-5 at P11-13, P30, P60, P90 and several control non-dystrophic rats (Long Evans and Sprague–Dawley) at P60, were studied anatomically by immunohistochemistry for various cell and synaptic markers, and by PNA and TUNEL label.- S334ter line-3 exhibited the fastest rate of degeneration with an early loss of photoreceptors, with 1–2 layers remaining at P30, and only cones left at P60. Line-5 had 4–5 layers left at P30, and very few rods left at P60-90. In both lines, horizontal cell processes (including dendrites and axon) were diminished at P11-13, showing gaps in the outer plexiform layer (OPL) at P60, and at P90, almost no terminal tips could be seen. Bipolar cells showed a retraction of their dendrites forming clusters along the OPL. Synaptic terminals of A-II amacrine cells in the IPL lost most of their parvalbumin-immunoreactivity. The apoptosis rate was different in both lines. Line-3 rats showed many photoreceptors affected at P11, occupying the innermost part of the outer nuclear layer. Line-5 showed a lower number of apoptotic cells within the same location at P13. In summary, the S334ter line-3 rat has a faster progression of degeneration than line-5. The horizontal and bipolar terminals are already affected at P11-P13 in both models. Apoptosis is related to the mutated rhodopsin transgene; the first photoreceptor cells affected are those close to the OPL. |
Patrocinador/es: | This study was supported by Ministerio de Ciencia e Innovación BFU2009-07793/BFI, RETICS RD07/0062/0012, Fundaluce, ONCE, Fundación Médica Mutua Madrileña to NC Ministerio de Sanidad, FIS PS09/01854 to IP; and an anonymous sponsor, Foundation Fighting Blindness [MJS, RBA]; and NIH EY03040 [Doheny vivarium core grant]. |
URI: | http://hdl.handle.net/10045/16716 |
ISSN: | 0014-4835 (Print) | 1096-0007 (Online) |
DOI: | 10.1016/j.exer.2010.12.001 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.exer.2010.12.001 |
Aparece en las colecciones: | INV - NEUROVIS - Artículos de Revistas |
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