Mutations at pipX suppress lethality of PII-deficient mutants of Synechococcus elongatus PCC 7942
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Título: | Mutations at pipX suppress lethality of PII-deficient mutants of Synechococcus elongatus PCC 7942 |
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Autor/es: | Espinosa, Javier | Castells Rico, Miguel Ángel | Laichoubi, Karim Boumediene | Contreras, Asunción |
Grupo/s de investigación o GITE: | Transducción de Señales en Bacterias |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Cyanobacteria |
Área/s de conocimiento: | Genética |
Fecha de creación: | 2009 |
Fecha de publicación: | ago-2009 |
Editor: | American Society for Microbiology |
Cita bibliográfica: | ESPINOSA MANZANO, Javier, et al. “Mutations at pipX suppress lethality of PII-deficient mutants of Synechococcus elongatus PCC 7942”. Journal of Bacteriology. Vol. 191, No. 15 (Aug. 2009). ISSN 0021-9193, pp. 4863-4869 |
Resumen: | The PII proteins are found in all three domains of life as key integrators of signals reflecting the balance of nitrogen and carbon. Genetic inactivation of PII proteins is typically associated with severe growth defects or death. However, the molecular basis of these defects depends on the specific functions of the proteins with which PII proteins interact to regulate nitrogen metabolism in different organisms. In Synechococcus elongatus PCC 7942, where PII forms complexes with the NtcA coactivator PipX, attempts to engineer PII-deficient strains failed in a wild-type background but were successful in pipX null mutants. Consistent with the idea that PII is essential to counteract the activity of PipX, four different spontaneous mutations in the pipX gene were found in cultures in which glnB had been genetically inactivated. |
Patrocinador/es: | This work was supported by grants BFU2006-12424 and ACOMP06/083. M.A.C. is the recipient of a predoctoral fellowship from the Universidad de Alicante. |
URI: | http://hdl.handle.net/10045/15132 |
ISSN: | 0021-9193 (Print) | 1098-5530 (Online) |
DOI: | 10.1128/JB.00557-09 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1128/JB.00557-09 |
Aparece en las colecciones: | INV - TSB - Artículos de Revistas |
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