Isolation and recrystallization of epicuticular waxes from Sorbus and Cotoneaster leaves
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Título: | Isolation and recrystallization of epicuticular waxes from Sorbus and Cotoneaster leaves |
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Autor/es: | Ganeva, Tsveta | Stefanova, Miroslava | Koleva, Dimitrina | Ríos, Segundo |
Grupo/s de investigación o GITE: | Biodiversidad y Biotecnología aplicadas a la Biología de la Conservación |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias Ambientales y Recursos Naturales | Universidad de Alicante. Centro Iberoamericano de la Biodiversidad |
Palabras clave: | Rosaceae | Epidermis | Wax extraction | Freeze-embedding | Recrystallization |
Fecha de publicación: | 27-oct-2015 |
Editor: | De Gruyter |
Cita bibliográfica: | Open Life Sciences. 2015, 10: 497-504. https://doi.org/10.1515/biol-2015-0051 |
Resumen: | Wax morphology and chemical composition are widely accepted to be important for the protective properties of the leaf’s surface and also valuable characteristics in plant systematics. The leaves of Sorbus domestica L. and Cotoneaster granatensis Boiss., species of two large genera with intricate taxonomy referred to subtribe Pyrinae, Rosaceae (formerly subfamily Maloideae), were studied by scanning electron microscope (SEM) and performing different methods of wax isolation. The aim of the study was to acquire a suitable, cost and time effective method for wax removal. Chloroform and methanol extractions and freeze-embedding method for direct isolation of the wax crystals were applied. Immersing the leaves for 3 minutes in chloroform was sufficient to extract the waxes whereas the efficiency of the methanol solvent was lower. Wax layers with well preserved structures of the crystals from both upper and lower epidermis were successfully transferred to artificial surfaces. The recrystallization experiment demonstrated that waxes from chloroform extracts could recrystallize in in vitro conditions on artificial surfaces. The crystals showed same micromorphology as on the intact leaves. Results of this study could be applied in further analytical researches of the waxes of S. domestica and C. granatensis or other species of the subtribe Pyrinae. |
Patrocinador/es: | This work was funded by project of the Ministry of Education and Science, Bulgaria, BG051PO001-3.3.05-0001. The publication of this study was financially supported by project BG051PO001-3.3.06 – 0045 |
URI: | http://hdl.handle.net/10045/141780 |
ISSN: | 2391-5412 |
DOI: | 10.1515/biol-2015-0051 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2015 Tsveta Ganeva et al., licensee De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1515/biol-2015-0051 |
Aparece en las colecciones: | INV - BBaBC - Artículos de Revistas |
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Ganeva_etal_2015_OpenLifeSci.pdf | 1,57 MB | Adobe PDF | Abrir Vista previa | |
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