Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942
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Título: | Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942 |
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Autor/es: | Llop Estevez, Antonio | Labella, Jose I. | Borisova, Marina | Forchhammer, Karl | Selim, Khaled A. | Contreras, Asunción |
Grupo/s de investigación o GITE: | Transducción de Señales en Bacterias |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Cyanobacteria | Polyphosphate | Stringent response | Nitrogen regulation | PipX | PipY | (p)ppGpp |
Fecha de publicación: | 16-mar-2023 |
Editor: | Frontiers Media |
Cita bibliográfica: | Llop A, Labella JI, Borisova M, Forchhammer K, Selim KA and Contreras A (2023) Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942. Front. Microbiol. 14:1141775. doi: 10.3389/fmicb.2023.1141775 |
Resumen: | The cyanobacterial protein PipY belongs to the Pyridoxal-phosphate (PLP)-binding proteins (PLPBP/COG0325) family of pyridoxal-phosphate-binding proteins, which are represented in all three domains of life. These proteins share a high degree of sequence conservation, appear to have purely regulatory functions, and are involved in the homeostasis of vitamin B6 vitamers and amino/keto acids. Intriguingly, the genomic context of the pipY gene in cyanobacteria connects PipY with PipX, a protein involved in signaling the intracellular energy status and carbon-to-nitrogen balance. PipX regulates its cellular targets via protein–protein interactions. These targets include the PII signaling protein, the ribosome assembly GTPase EngA, and the transcriptional regulators NtcA and PlmA. PipX is thus involved in the transmission of multiple signals that are relevant for metabolic homeostasis and stress responses in cyanobacteria, but the exact function of PipY is still elusive. Preliminary data indicated that PipY might also be involved in signaling pathways related to the stringent stress response, a pathway that can be induced in the unicellular cyanobacterium Synechococcus elongatus PCC7942 by overexpression of the (p)ppGpp synthase, RelQ. To get insights into the cellular functions of PipY, we performed a comparative study of PipX, PipY, or RelQ overexpression in S. elongatus PCC7942. Overexpression of PipY or RelQ caused similar phenotypic responses, such as growth arrest, loss of photosynthetic activity and viability, increased cell size, and accumulation of large polyphosphate granules. In contrast, PipX overexpression decreased cell length, indicating that PipX and PipY play antagonistic roles on cell elongation or cell division. Since ppGpp levels were not induced by overexpression of PipY or PipX, it is apparent that the production of polyphosphate in cyanobacteria does not require induction of the stringent response. |
Patrocinador/es: | This work was supported by grants PID2020-118816GB-I00 from the Spanish Government (MICINN) and VIGROB-126/21 from the University of Alicante to Asunción Contreras, PROMETEO/2021/057 to Francisco Martínez-Mojica from the Generalitat Valenciana, EXC 2124–390838134 of the DFG to Karl Forchhammer and Khaled A. Selim, the Federal Ministry of Education and Research (BMBF), the Baden-Württemberg Ministry of Science (PRO-SELIM-2022-14), 398967434-TRR261 from the DFG 832 and EXC2124 (CMFI) to Christoph Mayer. Antonio Llop was the recipient of a Santander-UA fellowship (BOUA/2021). |
URI: | http://hdl.handle.net/10045/133132 |
ISSN: | 1664-302X |
DOI: | 10.3389/fmicb.2023.1141775 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2023 Llop, Labella, Borisova, Forchhammer, Selim and Contreras. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3389/fmicb.2023.1141775 |
Aparece en las colecciones: | INV - TSB - Artículos de Revistas |
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