Physiological and Molecular Characterization of the Differential Response of Broccoli (Brassica oleracea var. Italica) Cultivars Reveals Limiting Factors for Broccoli Tolerance to Drought Stress
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Título: | Physiological and Molecular Characterization of the Differential Response of Broccoli (Brassica oleracea var. Italica) Cultivars Reveals Limiting Factors for Broccoli Tolerance to Drought Stress |
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Autor/es: | Chevilly, Sergio | Dolz-Edo, Laura | López-Nicolás, José M. | Morcillo Juliá, Luna | Vilagrosa, Alberto | Yenush, Lynne | Mulet, José M. |
Grupo/s de investigación o GITE: | Gestión de Ecosistemas y de la Biodiversidad (GEB) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ecología | CEAM (Centro de Estudios Ambientales del Mediterráneo) |
Palabras clave: | Broccoli | Drought stress | Plant hormones | Essential amino acids | Primary metabolites |
Área/s de conocimiento: | Ecología |
Fecha de publicación: | 27-ago-2021 |
Editor: | American Chemical Society |
Cita bibliográfica: | Journal of Agricultural and Food Chemistry. 2021, 69(35): 10394-10404. https://doi.org/10.1021/acs.jafc.1c03421 |
Resumen: | Broccoli is a cruciferous crop rich in health-promoting metabolites. Due to several factors, including anthropogenic global warming, aridity is increasing in many cultivation areas. There is a great demand to characterize the drought response of broccoli and use this knowledge to develop new cultivars able to maintain yield under water constraints. The aim of this study is to characterize the drought response at the physiological and molecular level of different broccoli (Brassica oleracea L. var. Italica Plenck) cultivars, previously characterized as drought-sensitive or drought-tolerant. This approach aims to identify different traits, which can constitute limiting factors for drought stress tolerance in broccoli. For this purpose, we have compared several physiological parameters and the complete profiles of amino acids, primary metabolites, hormones, and ions of drought-tolerant and drought-sensitive cultivars under stress and control conditions. We have found that drought-tolerant cultivars presented higher levels of methionine and abscisic acid and lower amounts of urea, quinic acid, and the gluconic acid lactone. Interestingly, we have also found that a drought treatment increases the levels of most essential amino acids in leaves and in florets. Our results have established physiological and molecular traits useful as distinctive markers to predict drought tolerance in broccoli or which could be reliably used for breeding new cultivars adapted to water scarcity. We have also found that a drought treatment increases the content of essential amino acids in broccoli. |
Patrocinador/es: | The authors thank Prof. Angel Maquieira for generously providing the technical equipment required for ion determination. S.C.’s work was funded by Grant FPU19/01977 from the Spanish Ministerio de Universidades. This work was funded by the RTC-2017-6468-2-AR project (APROXIMACIONES MOLECULARES PARA INCREMENTAR LA TOLERANCIA A SALINIDAD Y SEQUíA DEL BRÓCOLI) awarded by the “Agencia estatal de Investigación”. L.M. was supported by the Spanish MICINN (PTA2019-018094). Activities of L.M. and A.V. were funded by the Prometeu program (IMAGINA project, PROMETEU/2019/110). The CEAM foundation is funded by Generalitat Valenciana. The authors thank SAKATA for the generous gift of plant material. |
URI: | http://hdl.handle.net/10045/117582 |
ISSN: | 0021-8561 (Print) | 1520-5118 (Online) |
DOI: | 10.1021/acs.jafc.1c03421 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 American Chemical Society. Creative Commons Attribution 4.0 International License (CC BY 4.0) |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1021/acs.jafc.1c03421 |
Aparece en las colecciones: | INV - GEB - Artículos de Revistas |
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Chevilly_etal_2021_JAgricFoodChem.pdf | 6,42 MB | Adobe PDF | Abrir Vista previa | |
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