Towards the Elucidation of Assimilative nasABC Operon Transcriptional Regulation in Haloferax mediterranei
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Título: | Towards the Elucidation of Assimilative nasABC Operon Transcriptional Regulation in Haloferax mediterranei |
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Autor/es: | Pastor-Soler, Sandra | Camacho, Mónica | Bautista, Vanesa | Bonete, María-José | Esclapez, Julia |
Grupo/s de investigación o GITE: | Biotecnología de Extremófilos (BIOTECEXTREM) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Palabras clave: | Haloarchaea | Nitrogen assimilation | Transcriptional regulation | DNA–protein pull-down |
Área/s de conocimiento: | Bioquímica y Biología Molecular |
Fecha de publicación: | 22-abr-2021 |
Editor: | MDPI |
Cita bibliográfica: | Pastor-Soler S, Camacho M, Bautista V, Bonete M-J, Esclapez J. Towards the Elucidation of Assimilative nasABC Operon Transcriptional Regulation in Haloferax mediterranei. Genes. 2021; 12(5):619. https://doi.org/10.3390/genes12050619 |
Resumen: | The assimilatory pathway of the nitrogen cycle in the haloarchaeon Haloferax mediterranei has been well described and characterized in previous studies. However, the regulatory mechanisms involved in the gene expression of this pathway remain unknown in haloarchaea. This work focuses on elucidating the regulation at the transcriptional level of the assimilative nasABC operon (HFX_2002 to HFX_2004) through different approaches. Characterization of its promoter region using β-galactosidase as a reporter gene and site-directed mutagenesis has allowed us to identify possible candidate binding regions for a transcriptional factor. The identification of a potential transcriptional regulator related to nitrogen metabolism has become a real challenge due to the lack of information on haloarchaea. The investigation of protein–DNA binding by streptavidin bead pull-down analysis combined with mass spectrometry resulted in the in vitro identification of a transcriptional regulator belonging to the Lrp/AsnC family, which binds to the nasABC operon promoter (p.nasABC). To our knowledge, this study is the first report to suggest the AsnC transcriptional regulator as a powerful candidate to play a regulatory role in nasABC gene expression in Hfx. mediterranei and, in general, in the assimilatory nitrogen pathway. |
Patrocinador/es: | This research was funded by Universidad de Alicante, VIGROB-016. |
URI: | http://hdl.handle.net/10045/114449 |
ISSN: | 2073-4425 |
DOI: | 10.3390/genes12050619 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/genes12050619 |
Aparece en las colecciones: | INV - BIOTECEXTREM - Artículos de Revistas |
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Pastor-Soler_etal_2021_Genes.pdf | 2,13 MB | Adobe PDF | Abrir Vista previa | |
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