Bisphenol-S and Bisphenol-F alter mouse pancreatic β-cell ion channel expression and activity and insulin release through an estrogen receptor ERβ mediated pathway
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Título: | Bisphenol-S and Bisphenol-F alter mouse pancreatic β-cell ion channel expression and activity and insulin release through an estrogen receptor ERβ mediated pathway |
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Autor/es: | Marroquí, Laura | Martinez-Pinna, Juan | Castellano-Muñoz, Manuel | Dos Santos, Reinaldo S. | Medina-Gali, Regla M. | Soriano, Sergi | Quesada, Iván | Gustafsson, Jan-Ake | Encinar, José A. | Nadal, Ángel |
Grupo/s de investigación o GITE: | Fisiología Neuroendocrina (FINE) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Bisphenol | Islet of langerhans | Endocrine disrupting chemicals | Estrogen receptors | Molecular dynamics simulation |
Área/s de conocimiento: | Fisiología |
Fecha de publicación: | feb-2021 |
Editor: | Elsevier |
Cita bibliográfica: | Chemosphere. 2021, 265: 129051. https://doi.org/10.1016/j.chemosphere.2020.129051 |
Resumen: | Bisphenol-S (BPS) and Bisphenol-F (BPF) are current Bisphenol-A (BPA) substitutes. Here we used pancreatic β-cells from wild type (WT) and estrogen receptor β (ERβ) knockout (BERKO) mice to investigate the effects of BPS and BPF on insulin secretion, and the expression and activity of ion channels involved in β-cell function. BPS or BPF rapidly increased insulin release and diminished ATP-sensitive K+ (KATP) channel activity. Similarly, 48 h treatment with BPS or BPF enhanced insulin release and decreased the expression of several ion channel subunits in β-cells from WT mice, yet no effects were observed in cells from BERKO mice. PaPE-1, a ligand designed to preferentially trigger extranuclear-initiated ER pathways, mimicked the effects of bisphenols, suggesting the involvement of extranuclear-initiated ERβ pathways. Molecular dynamics simulations indicated differences in ERβ ligand-binding domain dimer stabilization and solvation free energy among different bisphenols and PaPE-1. Our data suggest a mode of action involving ERβ whose activation alters three key cellular events in β-cell, namely ion channel expression and activity, and insulin release. These results may help to improve the hazard identification of bisphenols. |
Patrocinador/es: | This work was supported by BPU2017-86579-R (AN) and BFU2016-77125-R (IQ) and RTI2018-096724-B-C21 (JAE) supported by FEDER /Ministerio de Ciencia e Innovación-Agencia Estatal de Investigación, Spain. PROMETEO/2020/006 (AN), PROMETEO/2016/006 (JAE) and SEJI/2018/023 (LM) supported by Generalitat Valenciana, Spain. J-AG was supported by the Robert A. Welch Foundation (E 0004), USA. CIBERDEM is an initiative of the Instituto de Salud Carlos III, Spain. |
URI: | http://hdl.handle.net/10045/112363 |
ISSN: | 0045-6535 (Print) | 1879-1298 (Online) |
DOI: | 10.1016/j.chemosphere.2020.129051 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2020 Elsevier Ltd. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1016/j.chemosphere.2020.129051 |
Aparece en las colecciones: | INV - FINE - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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Marroqui_etal_2021_Chemosphere_final.pdf | Versión final (acceso restringido) | 3,91 MB | Adobe PDF | Abrir Solicitar una copia |
Marroqui_etal_2021_Chemosphere_accepted.pdf | Accepted Manuscript (acceso abierto) | 16,23 MB | Adobe PDF | Abrir Vista previa |
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