Influence of in vitro capacitation time on structural and functional human sperm parameters
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Título: | Influence of in vitro capacitation time on structural and functional human sperm parameters |
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Autor/es: | Sáez Espinosa, Paula | Huerta-Retamal, Natalia | Robles-Gómez, Laura | Avilés Sánchez, Manuel | Aizpurua, Jon | Velasco-Ruiz, Irene | Romero, Alejandro | Gómez-Torres, María José |
Grupo/s de investigación o GITE: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Biotecnología |
Palabras clave: | Acrosome reaction | Capacitation | DNA fragmentation | Glycocalyx | Lectin-binding sites | Membrane integrity | Protein phosphorylation | Sperm selection |
Área/s de conocimiento: | Biología Celular |
Fecha de publicación: | 2020 |
Editor: | Wolters Kluwer - Medknow |
Cita bibliográfica: | Asian Journal of Andrology. 2020, 22: 447-453. https://doi.org/10.4103/aja.aja_104_19 |
Resumen: | A cascade of dramatic physiological events is linked to the sperm acrosome reaction and binding to the oocyte's zona pellucida during human sperm capacitation. However, structural and functional sperm changes during capacitation currently remain poorly defined. Here, we performed a multibiomarker approach based on the utilization of sperm concentration, motility, viability, morphology, acrosome reaction, tyrosine phosphorylation, DNA fragmentation, and lectin-binding sites to analyze the impact caused by swim-up selection times (uncapacitated, 1 h capacitated, and 4 h capacitated) on sperm function and structure in normozoospermic samples. We found that a 4 h swim-up capacitation increased sperm quality, because a large number of cells with normal morphology and lower DNA fragmentation rates were recovered. Furthermore, the long-term capacitation induced a higher percentage of cells with tyrosine phosphorylation of the principal piece as well as a redistribution of lectin-binding sites. Overall, the multivariate biomarkers analyzed showed a less variable distribution on spermatozoa recovered after 4 h capacitation than that with the shorter capacitation time. These findings stress the importance of capacitation time as a relevant factor in sperm quality with potential biological reproductive implications both for basic research and in assisted reproduction techniques. |
Patrocinador/es: | This research was funded by the Human Fertility Chair, the Department of Biotechnology of the University of Alicante (VIGROB-186), and the project of the Spanish Ministry of Economy and Competitiveness (AGL2015-70159-P). |
URI: | http://hdl.handle.net/10045/108674 |
ISSN: | 1008-682X (Print) | 1745-7262 (Online) |
DOI: | 10.4103/aja.aja_104_19 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © The Author(s) (2019) This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.4103/aja.aja_104_19 |
Aparece en las colecciones: | INV - Grupo de Inmunología - Artículos de Revistas |
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