Role of the Synechococcus PCC 7942 nitrogen regulator protein PipX in NtcA-controlled processes
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Título: | Role of the Synechococcus PCC 7942 nitrogen regulator protein PipX in NtcA-controlled processes |
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Autor/es: | Espinosa, Javier | Forchhammer, Karl | Contreras, Asunción |
Grupo/s de investigación o GITE: | Transducción de Señales en Bacterias |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universität Giessen. Institut für Mikrobiologie und Molekularbiologie |
Palabras clave: | Synechococcus PCC 7942 | Nitrogen regulator | Protein | PipX | NtcA |
Área/s de conocimiento: | Genética |
Fecha de creación: | 19-oct-2006 |
Fecha de publicación: | 2007 |
Editor: | Society for General Microbiology |
Cita bibliográfica: | ESPINOSA MANZANO, Javier; FORCHHAMMER, Karl; CONTRERAS DE VERA, Asunción. “Role of the Synechococcus PCC 7942 nitrogen regulator protein PipX in NtcA-controlled processes”. Microbiology. 153 (2007). ISSN 1350-0872, pp. 711-718 |
Resumen: | The Synechococcus sp. PCC 7942 nitrogen regulator PipX interacts in a 2-oxoglutarate-dependent manner with the global nitrogen transcription factor NtcA and the signal transduction protein PII. In vivo, PipX is involved in the NtcA-dependent induction of glnB and glnN genes. To further investigate the extent to which PipX is involved in global nitrogen control, the effect of pipX inactivation on various nitrogen-regulated processes was determined. The PipX-deficient mutant was able to use nitrate as a nitrogen source and to efficiently inhibit the nitrate transport upon ammonium addition but showed decreased nitrate and nitrite reductase activities and a delay in the induction of nitrate utilization after transfer of cultures from ammonium- to nitrate-containing media. In contrast to the wild-type, glutamine synthetase activity was not upregulated upon depletion of combined nitrogen from cultures of the mutant strain. Inactivation of pipX impaired induction of nblA and delayed phycobilisome degradation, but did not affect recovery of nitrogen-deprived cultures. Taken together, the results indicate that PipX interacts with NtcA to facilitate efficient acclimation of cyanobacteria to conditions of nitrogen limitation. |
Patrocinador/es: | Ministerio de Educación y Ciencia grants BMC2002-01156 and BFU2005-02231; Generalitat Valenciana grant GV04B-525; Deutsche Forschungsgemeinschaft grant Fo195/4. |
URI: | http://hdl.handle.net/10045/10341 |
ISSN: | 1350-0872 (Print) | 1465-2080 (Online) |
DOI: | 10.1099/mic.0.2006/003574-0 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | This is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://mic.sgmjournals.org, and is freely available without a subscription. |
Revisión científica: | si |
Aparece en las colecciones: | INV - TSB - Artículos de Revistas |
Archivos en este ítem:
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Espinosaetal2.pdf | Versión revisada (acceso libre) | 339,89 kB | Adobe PDF | Abrir Vista previa |
Espinosa_Role.pdf | Versión final (acceso restringido) | 219,24 kB | Adobe PDF | Abrir Solicitar una copia |
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