Unveiling viral–host interactions within the ‘microbial dark matter’
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Title: | Unveiling viral–host interactions within the ‘microbial dark matter’ |
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Authors: | Martinez-Garcia, Manuel | Santos, Fernando | Moreno-Paz, Mercedes | Parro, Víctor | Anton, Josefa |
Research Group/s: | Ecología Microbiana Molecular |
Center, Department or Service: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Keywords: | Viruses | Hosts | Interactions | Microbial dark matter |
Knowledge Area: | Microbiología |
Issue Date: | 14-Aug-2014 |
Publisher: | Macmillan Publishers |
Citation: | Nature Communications. 2014, 5: 4542. doi:10.1038/ncomms5542 |
Abstract: | Viruses control natural microbial communities. Identification of virus–host pairs relies either on their cultivation or on metagenomics and tentative assignment based on genomic signatures. Both approaches have severe drawbacks when aiming to target such pairs within the uncultured majority. Here we present an unambiguous way to assign viruses to hosts that does not rely on any previous information about either of them nor requires their cultivation. First, genomic contents of individual cells present in an environmental sample are retrieved by means of single-cell genomic technologies. Then, individual cell genomes are hybridized against a set of individual viral genomes from the same sample, previously immobilized on a microarray. Infected cells will yield positive hybridization as they carry viral genomes, which can be then sequenced and characterized. Using this method, we pinpoint viruses infecting the ubiquitous hyperhalophilic Nanohaloarchaeota, included in the so-called ‘microbial dark matter’ (the uncultured fraction of the microbial world). |
Sponsor: | This work was supported by the projects CGL2012-39627-C03-01 (to J.A.) and AYA2011-24803 (to V.P.) of the Spanish Ministry of Science and Innovation, which are co-financed with FEDER support from the European Union. |
URI: | http://hdl.handle.net/10045/47145 |
ISSN: | 2041-1723 |
DOI: | 10.1038/ncomms5542 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2014 Macmillan Publishers Limited |
Peer Review: | si |
Publisher version: | http://dx.doi.org/10.1038/ncomms5542 |
Appears in Collections: | INV - EMM - Artículos de Revistas |
Files in This Item:
File | Description | Size | Format | |
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2014-Nat-Com-Assigning-viruses-to-hosts.pdf | Versión final (acceso restringido) | 546,16 kB | Adobe PDF | Open Request a copy |
2014-Nat-Com-Assigning-viruses-to-hosts_revised.docx | Versión revisada (acceso abierto) | 104,78 kB | Microsoft Office Word 2007 document | Open |
Fig1.tif | Figure 1. Experimental design used to assign viruses to hosts in natural assemblages | 1,15 MB | TIFF | Open |
Fig2.tif | Figure 2. Virus NHV-1 infecting nanohaloarchaeon host D14 | 1,86 MB | TIFF | Open |
Fig3.tif | Figure 3. Principal component analyses (PCAs) of the dinucleotide frequency signatures of viruses and hosts | 6,14 MB | TIFF | Open |
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