Retinal microglia are activated by systemic fungal infection
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http://hdl.handle.net/10045/37207
Title: | Retinal microglia are activated by systemic fungal infection |
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Authors: | Maneu, Victoria | Noailles, Agustina | Megías, Javier | Gómez-Vicente, Violeta | Carpena, Núria | Gil, María Luisa | Gozalbo, Daniel | Cuenca, Nicolás |
Research Group/s: | Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) |
Center, Department or Service: | Universidad de Alicante. Departamento de Óptica, Farmacología y Anatomía | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Keywords: | Microglial activation | Retina | Infection | Candidiasis |
Knowledge Area: | Farmacología | Biología Celular |
Issue Date: | 15-May-2014 |
Publisher: | Association for Research in Vision and Ophthalmology (ARVO) |
Citation: | Investigative Ophthalmology & Visual Science. 2014, 55: 3578-3585. doi:10.1167/iovs.14-14051 |
Abstract: | Purpose: To determine whether systemic fungal infection could cause activation of retinal microglia and therefore could be potentially harmful for patients with retinal degenerative diseases. Methods: Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by (i) confocal immunofluorescence and (ii) flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII and anti-CD45 antibodies. Results: Systemic fungal infection causes activation of retinal microglia, with phenotypic changes in morphology, surface markers expression, and microglial re-location in retinal layers. Conclusions: As an excessive or prolonged microglial activation may lead to chronic inflammation with severe pathological side effects, causing or worsening the course of retinal dystrophies, a systemic infection may represent a risk factor to be considered in patients with ocular neurodegenerative diseases, such as diabetic retinopathy, glaucoma, age-related macular degeneration or retinitis pigmentosa. |
Sponsor: | This work was supported by projects grants from the Spanish Ministry of Economy and Competitiveness-FEDER BFU2012-36845, Instituto de Salud Carlos III RETICS RD12/0034/0010 and ONCE to Nicolás Cuenca and Instituto de Salud Carlos III PI080556 to Daniel Gozalbo. Violeta Gómez-Vicente is a MICINN-Juan de la Cierva post-doctoral fellow (JCI-2009-05224). |
URI: | http://hdl.handle.net/10045/37207 |
ISSN: | 0146-0404 (Print) | 1552-5783 (Online) |
DOI: | 10.1167/iovs.14-14051 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | Copyright © 2014 by Association for Research in Vision and Ophthalmology |
Peer Review: | si |
Publisher version: | http://dx.doi.org/10.1167/iovs.14-14051 |
Appears in Collections: | INV - NEUROVIS - Artículos de Revistas |
Files in This Item:
File | Description | Size | Format | |
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2014_Maneu_etal_IOVS.pdf | Versión revisada (acceso abierto) | 564,08 kB | Adobe PDF | Open Preview |
2014_Maneu_etal_IOVS_final.pdf | Versión final (acceso restringido) | 743,11 kB | Adobe PDF | Open Request a copy |
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