Sequencing and functional analysis of the genome of a nematode egg-parasitic fungus, Pochonia chlamydosporia
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Title: | Sequencing and functional analysis of the genome of a nematode egg-parasitic fungus, Pochonia chlamydosporia |
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Authors: | Larriba Tornel, Eduardo | Jaime, Maria DLA | Carbonell Caballero, José | Conesa, Ana | Dopazo, Joaquín | Nislow, Corey | Martín-Nieto, José | Lopez-Llorca, Luis Vicente |
Research Group/s: | Fitopatología | Genética Humana y de Mamíferos (GHM) |
Center, Department or Service: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Keywords: | Pochonia chlamydosporia | Genome sequencing | Nematophagous fungus | Endophyte | Hydrolytic enzymes | Gene ontology |
Knowledge Area: | Botánica | Genética |
Issue Date: | 13-Feb-2014 |
Publisher: | Elsevier |
Citation: | Fungal Genetics and Biology. 2014, 65: 69-80. doi:10.1016/j.fgb.2014.02.002 |
Abstract: | Pochonia chlamydosporia is a worldwide-distributed soil fungus with a great capacity to infect and destroy the eggs and kill females of plant-parasitic nematodes. Additionally, it has the ability to colonize endophytically roots of economically-important crop plants, thereby promoting their growth and eliciting plant defenses. This multitrophic behavior makes P. chlamydosporia a potentially useful tool for sustainable agriculture approaches. We sequenced and assembled ∼41 Mb of P. chlamydosporia genomic DNA and predicted 12,122 gene models, of which many were homologous to genes of fungal pathogens of invertebrates and fungal plant pathogens. Predicted genes (65%) were functionally annotated according to Gene Ontology, and 16% of them found to share homology with genes in the Pathogen Host Interactions (PHI) database. The genome of this fungus is highly enriched in genes encoding hydrolytic enzymes, such as proteases, glycoside hydrolases and carbohydrate esterases. We used RNA-Seq technology in order to identify the genes expressed during endophytic behavior of P. chlamydosporia when colonizing barley roots. Functional annotation of these genes showed that hydrolytic enzymes and transporters are expressed during endophytism. This structural and functional analysis of the P. chlamydosporia genome provides a starting point for understanding the molecular mechanisms involved in the multitrophic lifestyle of this fungus. The genomic information provided here should also prove useful for enhancing the capabilities of this fungus as a biocontrol agent of plant-parasitic nematodes and as a plant growth-promoting organism. |
Sponsor: | This research was funded by the Spanish Ministry of Science and Innovation Grant AGL2011-29297. |
URI: | http://hdl.handle.net/10045/35668 |
ISSN: | 1087-1845 (Print) | 1096-0937 (Online) |
DOI: | 10.1016/j.fgb.2014.02.002 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Peer Review: | si |
Publisher version: | http://dx.doi.org/10.1016/j.fgb.2014.02.002 |
Appears in Collections: | INV - Fitopatología - Artículos de Revistas INV - GHM - Artículos de Revistas |
Files in This Item:
File | Description | Size | Format | |
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2014_Larriba_etal_FGB.pdf | Accepted Manuscript (acceso abierto) | 4,47 MB | Adobe PDF | Open Preview |
2014_Larriba_etal_FGB_final.pdf | Versión final (acceso restringido) | 2,6 MB | Adobe PDF | Open Request a copy |
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