Pedro Roig, Laia, Camacho, Mónica, Bonete, María-José Haloferax mediterranei GlnK proteins are post-translationally modified by uridylylation Proteomics. 2013, 13(8): 1371-1374. doi:10.1002/pmic.201200465 URI: http://hdl.handle.net/10045/44400 DOI: 10.1002/pmic.201200465 ISSN: 1615-9853 (Print) Abstract: In this work we report for the first time a post-translational modification of PII homologues from the Archaea Domain. Haloferax mediterranei is the first haloarchaea whose PII proteins have been studied, it possesses two of them (GlnK1 and GlnK2), both encoded adjacent to a gene for the ammonia transporter Amt. An approach based on 2DE, anti-GlnK immunoblot and peptide mass fingerprint (MALDI-TOF-MS) of the reactive spots showed that GlnK proteins in H. mediterranei are post-translationally uridylylated. A third spot with lower pI suggests the existence of a non-descript post-translational modification in this protein family. Keywords:Archaea, GlnK, Haloferax, Microbiology, Post-translational modification, Uridylylation Wiley-VCH Verlag GmbH & Co. KGaA info:eu-repo/semantics/article