Insights into the antibiotic resistance dissemination in a wastewater effluent microbiome: bacteria, viruses and vesicles matter

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Title: Insights into the antibiotic resistance dissemination in a wastewater effluent microbiome: bacteria, viruses and vesicles matter
Authors: Maestre-Carballa, Lucia | Lluesma Gómez, Mónica | Angla Navarro, Andrea | Garcia-Heredia, Inmaculada | Martinez-Hernandez, Francisco | Martinez-Garcia, Manuel
Research Group/s: Ecología Microbiana Molecular
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Antibiotic resistance genes | Wastewater | Bacteria | Viruses | Vesicles
Knowledge Area: Microbiología
Issue Date: Dec-2019
Publisher: John Wiley & Sons
Citation: Environmental Microbiology. 2019, 21(12): 4582-4596. doi:10.1111/1462-2920.14758
Abstract: Wastewater treatment plants effluents are considered as hotspots for the dispersion of antibiotic resistance genes (ARGs) into natural ecosystems. The bacterial resistome (ARG collection in a metagenome) analyses have provided clues on antibacterial resistance dynamics. However, viruses and vesicles are frequently ignored. Here, we addressed the bacterial, viral and vesicle resistomes from a representative wastewater effluent in natural conditions and amended with polymyxin, which is used as a last resort antibiotic. Metagenomics showed that the natural prokaryotic resistome was vast (9000 ARG hits/Gb metagenome) and diverse, while viral resistome was two orders of magnitude lower (50 ARG hits/Gb metagenome) suggesting that viruses rarely encoded ARGs. After polymyxin amendment, data showed no ARG enrichment – including to polymyxin – in the microbiome. Remarkably, microbiomes responded to polymyxin with a vast release of putative vesicles (threefold increase compared with the control), which might be used as 'traps' to decrease the antibiotic concentration. Intriguingly, although polymyxin resistance genes (PRGs) were rare in the microbiome (0.018% of total ARG found), in the viral and vesicle fractions, PRGs were more abundant (0.5%–0.8% of total ARG found). Our data suggest that vesicles could have a more active role in the context of transmission of antibiotic resistances.
Sponsor: This work has been supported by Ministerio de Ciencia, Innovación y Universidades (ref. RTI2018-094248-B-I00), Generalitat Valenciana (refs. ACOM/2015/133 and ACIF/2015/332), and Gordon and Betty Moore Foundation (grant 5334).
ISSN: 1462-2912 (Print) | 1462-2920 (Online)
DOI: 10.1111/1462-2920.14758
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.
Peer Review: si
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