Homocysteine enhances superoxide anion release and NADPH oxidase assembly by human neutrophils: effects on MAPK activation and neutrophil migration
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/9731
Título: | Homocysteine enhances superoxide anion release and NADPH oxidase assembly by human neutrophils: effects on MAPK activation and neutrophil migration |
---|---|
Autor/es: | Álvarez Maqueda, Moisés | El Bekay, Rajaa | Monteseirín Mateo, Javier | Alba Jiménez, Gonzalo | Chacón Fernández, Pedro | Vega Rioja, Antonio | Santa María Pérez, Consuelo | Tejedo Huaman, Juan Rigoberto | Martín-Nieto, José | Bedoya Bergua, Francisco Javier | Pintado Sanjuan, Elizabeth | Sobrino Beneyto, Francisco |
Grupo/s de investigación o GITE: | Genética Humana y de Mamíferos |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular | Hospital Universitario Virgen Macarena. Servicio Regional de Inmunología y Alergia | Universidad de Sevilla. Departamento de Bioquímica, Bromatología y Toxicología |
Palabras clave: | Homocysteine | NADPH oxidase | Reactive oxygen species | MAPK | Neutrophils |
Área/s de conocimiento: | Genética | Bioquímica y Biología Molecular |
Fecha de creación: | 6-may-2003 |
Fecha de publicación: | feb-2004 |
Editor: | Elsevier |
Cita bibliográfica: | ÁLVAREZ MAQUEDA, Moisés, et al. "Homocysteine enhances superoxide anion release and NADPH oxidase assembly by human neutrophils: effects on MAPK activation and neutrophil migration". Atherosclerosis. Vol. 172, Issue 2 (Febr. 2004). ISSN 0021-9150, pp. 229-238 |
Resumen: | Hyperhomocysteinaemia has recently been recognized as a risk factor of cardiovascular disease. However, the action mechanisms of homocysteine (Hcy) are not well understood. Given that Hcy may be involved in the recruitment of monocytes and neutrophils to the vascular wall, we have investigated the role of Hcy in essential functions of human neutrophils. We show that Hcy increased superoxide anion (O2√−) release by neutrophils to the extracellular medium, and that this effect was inhibited by superoxide dismutase and diphenyleneiodonium (DPI), an inhibitor of NADPH oxidase activity. The enzyme from rat peritoneal macrophages displayed a similar response. These effects were accompanied by a time-dependent increased translocation of p47phox and p67phox subunits of NADPH oxidase to the plasma membrane. We also show that Hcy increased intracellular H2O2 production by neutrophils, that Hcy enhanced the activation and phosphorylation of mitogen-activated protein kinases (MAPKs), specifically p38-MAPK and ERK1/2, and that the migration of neutrophils was increased by Hcy. Present results are the first evidence that Hcy enhances the oxidative stress of neutrophils, and underscore the potential role of phagocytic cells in vascular wall injury through O2√− release in hyperhomocysteinaemia conditions. |
Patrocinador/es: | This work was financed by Grants from the Ministerio de Ciencia y Tecnología SAF/2000-117 (awarded to F.S.) and SAF/2000-161 (given to F.J.B.), and by a Grant from the Fundación SEIAC, Spain (awarded to J.M.). |
URI: | http://hdl.handle.net/10045/9731 |
ISSN: | 0021-9150 (Print) | 1879-1484 (Online) |
DOI: | 10.1016/j.atherosclerosis.2003.11.005 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.atherosclerosis.2003.11.005 |
Aparece en las colecciones: | INV - GHM - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Álvarez 2004 Atherosclerosis.pdf | Versión final (acceso restringido) | 330,92 kB | Adobe PDF | Abrir Solicitar una copia |
Todos los documentos en RUA están protegidos por derechos de autor. Algunos derechos reservados.