Characterization of calcineurin in human neutrophils: inhibitory effect of hydrogen peroxide on its enzyme activity and on NF-κB DNA binding
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Campo DC | Valor | Idioma |
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dc.contributor | Genética Humana y de Mamíferos | en |
dc.contributor.author | Carballo Álvarez, Modesto | - |
dc.contributor.author | Márquez, Gracia | - |
dc.contributor.author | Conde, Manuel | - |
dc.contributor.author | Martín-Nieto, José | - |
dc.contributor.author | Monteseirín Mateo, Javier | - |
dc.contributor.author | Conde Hernández, José | - |
dc.contributor.author | Pintado Sanjuan, Elizabeth | - |
dc.contributor.author | Sobrino Beneyto, Francisco | - |
dc.contributor.other | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | en |
dc.contributor.other | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular | en |
dc.contributor.other | Hospital Universitario Virgen Macarena. Servicio Regional de Inmunología y Alergia | en |
dc.date.accessioned | 2009-02-23T07:53:57Z | - |
dc.date.available | 2009-02-23T07:53:57Z | - |
dc.date.created | 1998-08-18 | - |
dc.date.issued | 1999-01-01 | - |
dc.identifier.citation | CARBALLO ÁLVAREZ, Modesto, et al. "Characterization of calcineurin in human neutrophils: inhibitory effect of hydrogen peroxide on its enzyme activity and on NF-κB DNA binding". Journal of Biological Chemistry. Vol. 274, No. 1 (Jan. 1999). ISSN 0021-9258, pp. 93-100 | en |
dc.identifier.issn | 0021-9258 (Print) | - |
dc.identifier.issn | 1083-351X (Online) | - |
dc.identifier.uri | http://hdl.handle.net/10045/9722 | - |
dc.description.abstract | We describe here a specific calcineurin activity in neutrophil lysates, which is dependent on Ca2+, inhibited by trifluoroperazine, and insensitive to okadaic acid. Immunoblotting experiments using a specific antiserum recognized both the A and B chains of calcineurin. Neutrophils treated with cyclosporin A or FK 506 showed a dose-dependent inhibition of calcineurin activity. The effect of oxidant compounds on calcineurin activity was also investigated. Neutrophils treated with hydrogen peroxide (H2O2), where catalase was inhibited with aminotriazole, exhibited a specific inhibition of calcineurin activity. However, the addition of reducing agents to neutrophil extracts partially reversed the inhibition caused by H2O2. A similar inhibitory effect of H2O2 on calcineurin activity was observed to occur in isolated lymphocytes. This is the first demonstration that redox agents modulate calcineurin activity in a cellular system. In addition, electrophoretic mobility shift assays revealed that lipopolysaccharide-induced activation of NF-κB in human neutrophils is inhibited by cell pretreatment with H2O2 in a dose-dependent manner. These data indicate that calcineurin activity regulates the functional activity of lipopolysaccharide-induced NF-κB/Rel proteins in human neutrophils. These data indicate a role of peroxides in the modulation of calcineurin activity and that the H2O2-dependent NF-κB inactivation in neutrophils occurs in concert with inhibition of calcineurin. | en |
dc.description.sponsorship | This work was supported in part by Fondo Investigaciones Sanitarias Grants 94/1484 and 97/1289 (to F. S.) and Grant 97/207 (to J. C.) and the Fundation of SEAIC of Spain. | en |
dc.language | eng | en |
dc.publisher | American Society for Biochemistry and Molecular Biology | en |
dc.rights | This research was originally published in Journal of Biological Chemistry. Carballo Álvarez, Modesto, et al. Characterization of calcineurin in human neutrophils: inhibitory effect of hydrogen peroxide on its enzyme activity and on NF-κB DNA binding. Journal of Biological Chemistry. 1999. 274:93-100. © the American Society for Biochemistry and Molecular Biology | - |
dc.subject | Calcineurin | en |
dc.subject | Human neutrophils | en |
dc.subject | Enzyme activity | en |
dc.subject | Hydrogen peroxide | en |
dc.subject.other | Genética | en |
dc.subject.other | Bioquímica y Biología Molecular | en |
dc.title | Characterization of calcineurin in human neutrophils: inhibitory effect of hydrogen peroxide on its enzyme activity and on NF-κB DNA binding | en |
dc.type | info:eu-repo/semantics/article | en |
dc.peerreviewed | si | en |
dc.identifier.doi | 10.1074/jbc.274.1.93 | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Aparece en las colecciones: | INV - GHM - Artículos de Revistas |
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