Transcriptomic analysis of Citrus clementina mandarin fruits maturation reveals a MADS-box transcription factor that might be involved in the regulation of earliness

Please use this identifier to cite or link to this item:
Información del item - Informació de l'item - Item information
Title: Transcriptomic analysis of Citrus clementina mandarin fruits maturation reveals a MADS-box transcription factor that might be involved in the regulation of earliness
Authors: Terol, Javier | Nueda, María José | Ventimilla, Daniel | Tadeo, Francisco | Talon, Manuel
Research Group/s: Bioquímica Aplicada/Applied Biochemistry (AppBiochem) | Sistemas Dinámicos y Estadística (SISDINEST)
Center, Department or Service: Universidad de Alicante. Departamento de Matemáticas
Keywords: Citrus | RNA-Seq | Fruit | Ripening | Earliness | Lateness | Transcription factor | MADS box
Knowledge Area: Estadística e Investigación Operativa
Issue Date: 31-Jan-2019
Publisher: Springer Nature
Citation: BMC Plant Biology. 2019, 19:47. doi:10.1186/s12870-019-1651-z
Abstract: Background: Harvest time is a relevant economic trait in citrus, and selection of cultivars with different fruit maturity periods has a remarkable impact in the market share. Generation of early- and late-maturing cultivars is an important target for citrus breeders, therefore, generation of knowledge regarding the genetic mechanisms controlling the ripening process and causing the early and late phenotypes is crucial. In this work we analyze the evolution of the transcriptome during fruit ripening in 3 sport mutations derived from the Fina clementine (Citrus clementina) mandarin: Clemenules (CLE), Arrufatina (ARR) and Hernandina (HER) that differ in their harvesting periods. CLE is considered a mid-season cultivar while ARR and HER are early- and late-ripening mutants, respectively. Results: We used RNA-Seq technology to carry out a time course analysis of the transcriptome of the 3 mutations along the ripening period. The results indicated that in these mutants, earliness and lateness during fruit ripening correlated with the advancement or delay in the expression of a set of genes that may be implicated in the maturation process. A detailed analysis of the transcription factors known to be involved in the regulation of fruit ripening identified a member of the MADS box family whose expression was lower in ARR, the early-ripening mutant, and higher in HER, the late-ripening mutant. The pattern of expression of this gene during the maturation period was basically contrary to those of the ethylene biosynthetic genes, SAM and ACC synthases and ACC oxidase. The gene was present in hemizygous dose in the early-ripening mutant. Conclusions: Our analysis provides new clues about the genetic control of fruit ripening in citrus and allowed the identification of a transcription factor that could be involved in the early phenotype.
Sponsor: Financial support was provided by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, (Ministerio de Economía, Industria y Competitividad e Innovación, Spain) trough grant # RTA-00071-C06–01.
ISSN: 1471-2229
DOI: 10.1186/s12870-019-1651-z
Language: eng
Type: info:eu-repo/semantics/article
Rights: © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.
Peer Review: si
Publisher version:
Appears in Collections:INV - GESTA - Artículos de Revistas
INV - AppBiochem - Artículos de Revistas
INV - SISDINEST - Artículos de Revistas

Files in This Item:
Files in This Item:
File Description SizeFormat 
Thumbnail2019_Terol_etal_BMCPlantBio.pdf3,7 MBAdobe PDFOpen Preview

This item is licensed under a Creative Commons License Creative Commons