The nitrogen regulator PipX acts in cis to prevent operon polarity

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Title: The nitrogen regulator PipX acts in cis to prevent operon polarity
Authors: Cantos, Raquel | Labella, Jose I. | Espinosa Manzano, Javier | Contreras, Asunción
Research Group/s: Transducción de Señales en Bacterias
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Cyanobacteria | Nitrogen regulator | PipX | Operon polarity
Knowledge Area: Genética
Issue Date: 20-Aug-2018
Publisher: Wiley
Citation: Environmental Microbiology Reports. 2019, 11(4): 495-507. doi:10.1111/1758-2229.12688
Abstract: Cyanobacteria, phototrophic organisms performing oxygenic photosynthesis, must adapt their metabolic processes to important environmental challenges, like those imposed by the succession of days and nights. Not surprisingly, certain regulatory proteins are found exclusively in this phylum. One of these unique factors, PipX, provides a mechanistic link between signals of carbon/nitrogen and of energy, transduced by the signalling protein PII, and the control of gene expression by the global nitrogen regulator NtcA. Here we report a new regulatory function of PipX: enhancement in cis of pipY expression, a gene encoding a universally conserved protein involved in amino/keto acid and Pyridoxal phosphate homeostasis. In Synechococcus elongatus and many other cyanobacteria these genes are expressed as a bicistronic pipXY operon. Despite being cis‐acting, polarity suppression by PipX is nevertheless reminiscent of the function of NusG paralogues typified by RfaH, which are non‐essential operon‐specific bacterial factors acting in trans to upregulate horizontally‐acquired genes. Furthermore, PipX and members of the NusG superfamily share a TLD/KOW structural domain, suggesting regulatory interactions of PipX with the translation machinery. Our results also suggest that the cis‐acting function of PipX is a sophisticated regulatory strategy for maintaining appropriate PipX–PipY stoichiometry.
Sponsor: The authors thank the Spanish Government (MINECO) for supporting grants BFU2012‐33364 and BFU2015‐66360‐P to A.C. and the University of Alicante for PhD fellowship (FPUUA59) to J.I.L.
URI: http://hdl.handle.net/10045/82813
ISSN: 1758-2229
DOI: 10.1111/1758-2229.12688
Language: eng
Type: info:eu-repo/semantics/article
Rights: © John Wiley & Sons, Inc.
Peer Review: si
Publisher version: https://doi.org/10.1111/1758-2229.12688
Appears in Collections:INV - TSB - Artículos de Revistas

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