Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets

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Title: Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets
Authors: Martínez-Peinado, Pascual | Pascual García, Sandra | Roche, Enrique | Sempere Ortells, José Miguel
Research Group/s: Grupo de Inmunología, Biología Celular y del Desarrollo
Center, Department or Service: Universidad de Alicante. Departamento de Biotecnología
Keywords: Mesenchymal stem cells | Immunomodulation | Lymphocyte subsets
Knowledge Area: Inmunología
Issue Date: 9-Sep-2018
Publisher: Hindawi Publishing Corporation
Citation: Journal of Immunology Research. Volume 2018, Article ID 7232717, 11 pages. doi:10.1155/2018/7232717
Abstract: Mesenchymal stem cells (MSC) are a widely used population in cell therapy for their ability to differentiate into distinct tissues and more lately, for their immunomodulatory properties. However, the use of heterogeneous populations could be responsible for the nondesired outcomes reflected in the literature. Here, we analyse the different capacities of five one-cell-derived MSC clones to exert their immunomodulation ex vivo. We assessed proliferation assays in cocultures of MSC clones and purified cluster of differentiation (CD)3+, CD4+, or CD8+ lymphocytes; analysed the regulatory T (Treg) cells fold change rate; determined the effects on viability of peripheral blood mononuclear cells (PBMC); and also measured the coculture cytokine profiles (Th1/Th2). Conditioned media (CM) of different clones were also used to perform both proliferation assays and to analyse Treg fold change. The five clones analysed in this work were able to generate heterogeneous environments. Different clones inhibited proliferation of CD3+ and CD4+ lymphocytes, with different intensities. Surprisingly, all clones promoted proliferation of CD8+ lymphocytes. Different MSC clones and their CM were able to increase the number of Treg with different intensities. Finally, different clones also promoted different effects on the viability of PBMC treated with ultraviolet light. Considering all these data together, it seems that different clones, even from the same donor, can promote a wide spectrum of responses from anti-inflammatory to proinflammatory character. This fact may be important to standardise the design of personalized cell therapy protocols, thus diminishing the aforementioned undesired outcomes existing nowadays in this type of therapies.
URI: http://hdl.handle.net/10045/79748
ISSN: 2314-8861 (Print) | 2314-7156 (Online)
DOI: 10.1155/2018/7232717
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2018 Pascual Martínez-Peinado et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Peer Review: si
Publisher version: https://doi.org/10.1155/2018/7232717
Appears in Collections:INV - Grupo de Inmunología - Artículos de Revistas

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