Tailoring tobacco hairy root metabolism for the production of stilbenes

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Title: Tailoring tobacco hairy root metabolism for the production of stilbenes
Authors: Hidalgo, Diego | Georgiev, Milen | Marchev, Andrey | Bru-Martinez, Roque | Cusidó, Rosa M. | Corchete, Purificación | Palazon, Javier
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Tobacco hairy root | Stilbenes
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 21-Dec-2017
Publisher: Springer Nature
Citation: Scientific Reports. 2017, 7: 17976. doi:10.1038/s41598-017-18330-w
Abstract: Tobacco hairy root (HR) cultures, which have been widely used for the heterologous production of target compounds, have an innate capacity to bioconvert exogenous t-resveratrol (t-R) into t-piceatannol (t-Pn) and t-pterostilbene (t-Pt). We established genetically engineered HR carrying the gene encoding stilbene synthase (STS) from Vitis vinifera and/or the transcription factor (TF) AtMYB12 from Arabidopsis thaliana, in order to generate a holistic response in the phenylpropanoid pathway and coordinate the up-regulation of multiple metabolic steps. Additionally, an artificial microRNA for chalcone synthase (amiRNA CHS) was utilized to arrest the normal flux through the endogenous chalcone synthase (CHS) enzyme, which would otherwise compete for precursors with the STS enzyme imported for the flux deviation. The transgenic HR were able to biosynthesize the target stilbenes, achieving a production of 40 μg L−1 of t-R, which was partially metabolized into t-Pn and t-Pt (up to 2.2 μg L−1 and 86.4 μg L−1, respectively), as well as its glucoside piceid (up to 339.7 μg L−1). Major metabolic perturbations were caused by the TF AtMYB12, affecting both primary and secondary metabolism, which confirms the complexity of biotechnological systems based on seed plant in vitro cultures for the heterologous production of high-value molecules.
Sponsor: This work has been supported by a grant from the Spanish Ministry of Science and Innovation (BIO2014-51861-R, BIO2017-82374-R), Generalitat de Catalunya (2014SGR215). Diego Hidalgo is a predoctoral fellow of Mexican CONACyT.
URI: http://hdl.handle.net/10045/72318
ISSN: 2045-2322
DOI: 10.1038/s41598-017-18330-w
Language: eng
Type: info:eu-repo/semantics/article
Rights: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Peer Review: si
Publisher version: http://dx.doi.org/10.1038/s41598-017-18330-w
Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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