Human sperm motility, capacitation and acrosome reaction are impaired by 2-arachidonoylglycerol endocannabinoid

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Title: Human sperm motility, capacitation and acrosome reaction are impaired by 2-arachidonoylglycerol endocannabinoid
Authors: Francou, María Manuela | Girela, Jose L. | Juan, Alba de | Ten Morro, Jorge | Bernabeu, Rafael | Juan Herrero, Joaquín de
Research Group/s: Biotecnología
Center, Department or Service: Universidad de Alicante. Departamento de Biotecnología
Keywords: Arosome reaction | Arachidonoylglycerol | Endocannabinoid | Noladin | Protein phosphorylation
Knowledge Area: Biología Celular
Issue Date: Dec-2017
Publisher: Histology and Histopathology
Citation: Histology and Histopathology. 2017, 32(12): 1351-1358. doi:10.14670/HH-11-911
Abstract: The endocannabinoids are cannabinoids synthesized by mammalian tissues. These compounds are closely related to the regulation of the male reproductive system. However, little is known about the effects produced by 2-arachidonoylglycerol (2AG) on in vitro human sperm functions. This study was undertaken to determine the effects produced by 2AG on fresh human sperm and in the capacitation technique. Semen samples from healthy young men were exposed to different concentrations of 2AG before and during capacitation technique. In this work, we have demonstrated that 2AG induces the spontaneous acrosome reaction and reduces progressive motility in fresh human sperm. During the capacitation technique, sperm becomes more sensitive to low concentrations of 2AG, triggering the acrosome reaction and inhibiting protein phosphorylation. In summary, 2AG affects the in vitro functionality of human sperm by reducing motility, inhibiting capacitation and triggering the acrosome reaction.
Sponsor: This project was supported by the Chair or Reproductive Medicine, University of Alicante -Instituto Bernabeu, Grant 4-12I to JDJ, as well as the Office of the Vice Chancellor for Research, Development and Innovation, University of Alicante, Spain, (Grant Vigrob-137 to JDJ). The project is part of first prize in the Merck Serono Award for Innovation, Quality and Image in Assisted Reproduction (iCIRA Award), granted in October 2011. JDJ was Head of Research.
ISSN: 0213-3911 | 1699-5848 (Internet)
DOI: 10.14670/HH-11-911
Language: eng
Type: info:eu-repo/semantics/article
Rights: © Histology and Histopathology
Peer Review: si
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