Chitosan Increases Tomato Root Colonization by Pochonia chlamydosporia and Their Combination Reduces Root-Knot Nematode Damage

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Título: Chitosan Increases Tomato Root Colonization by Pochonia chlamydosporia and Their Combination Reduces Root-Knot Nematode Damage
Autor/es: Escudero Benito, Nuria | Lopez-Moya, Federico | Ghahremani, Zahra | Zavala-González, Ernesto A. | Alaguero-Cordovilla, Aurora | Ros-Ibañez, Caridad | Lacasa, Alfredo | Sorribas, Francisco J. | Lopez-Llorca, Luis Vicente
Grupo/s de investigación o GITE: Fitopatología
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef"
Palabras clave: Endophytic colonization | Nematophagous fungi | Root-knot nematodes | Suppressive soil | Solanum lycopersicum
Área/s de conocimiento: Botánica
Fecha de publicación: 1-sep-2017
Editor: Frontiers Media
Cita bibliográfica: Escudero N, Lopez-Moya F, Ghahremani Z, Zavala-Gonzalez EA, Alaguero-Cordovilla A, Ros-Ibañez C, Lacasa A, Sorribas FJ and Lopez-Llorca LV (2017) Chitosan Increases Tomato Root Colonization by Pochonia chlamydosporia and Their Combination Reduces Root-Knot Nematode Damage. Front. Plant Sci. 8:1415. doi: 10.3389/fpls.2017.01415
Resumen: The use of biological control agents could be a non-chemical alternative for management of Meloidogyne spp. [root-knot nematodes (RKN)], the most damaging plant-parasitic nematodes for horticultural crops worldwide. Pochonia chlamydosporia is a fungal parasite of RKN eggs that can colonize endophytically roots of several cultivated plant species, but in field applications the fungus shows a low persistence and efficiency in RKN management. The combined use of P. chlamydosporia with an enhancer could help its ability to develop in soil and colonize roots, thereby increasing its efficiency against nematodes. Previous work has shown that chitosan enhances P. chlamydosporia sporulation and production of extracellular enzymes, as well as nematode egg parasitism in laboratory bioassays. This work shows that chitosan at low concentrations (up to 0.1 mg ml-1) do not affect the viability and germination of P. chlamydosporia chlamydospores and improves mycelial growth respect to treatments without chitosan. Tomato plants irrigated with chitosan (same dose limit) increased root weight and length after 30 days. Chitosan irrigation increased dry shoot and fresh root weight of tomato plants inoculated with Meloidogyne javanica, root length when they were inoculated with P. chlamydosporia, and dry shoot weight of plants inoculated with both P. chlamydosporia and M. javanica. Chitosan irrigation significantly enhanced root colonization by P. chlamydosporia, but neither nematode infection per plant nor fungal egg parasitism was affected. Tomato plants cultivated in a mid-suppressive (29.3 ± 4.7% RKN egg infection) non-sterilized clay loam soil and irrigated with chitosan had enhanced shoot growth, reduced RKN multiplication, and disease severity. Chitosan irrigation in a highly suppressive (73.7 ± 2.6% RKN egg infection) sterilized-sandy loam soil reduced RKN multiplication in tomato. However, chitosan did not affect disease severity or plant growth irrespective of soil sterilization. Chitosan, at an adequate dose, can be a potential tool for sustainable management of RKN.
Patrocinador/es: This research was funded by two grants from the Spanish Ministry of Economy and Competitiveness (AGL 2013-49040-C2-1-R and AGL2015-66833-R,) and by a Ph.D. fellowship from the University of Alicante to NE (UAFPU2011). Part of this work was filed for a patent (P201431399) by LL-L, FL-M, and NE as inventors.
ISSN: 1664-462X
DOI: 10.3389/fpls.2017.01415
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2017 Escudero, Lopez-Moya, Ghahremani, Zavala-Gonzalez, Alaguero-Cordovilla, Ros-Ibañez, Lacasa, Sorribas and Lopez-Llorca. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Revisión científica: si
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Aparece en las colecciones:INV - Fitopatología - Artículos de Revistas

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