Silybum marianum cell cultures stably transformed with Vitis vinifera stilbene synthase accumulate t-resveratrol in the extracellular medium after elicitation with methyl jasmonate or methylated β-cyclodextrins

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Title: Silybum marianum cell cultures stably transformed with Vitis vinifera stilbene synthase accumulate t-resveratrol in the extracellular medium after elicitation with methyl jasmonate or methylated β-cyclodextrins
Authors: Hidalgo, Diego | Martínez Márquez, Ascensión | Cusidó, Rosa M. | Bru-Martinez, Roque | Palazon, Javier | Corchete, Purificación
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Flavonolignans | Heterologous gene expression | Plant cell cultures | Resveratrol | Silybum marianum
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: Jun-2017
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
Citation: Engineering in Life Sciences. 2017, 17(6): 686-694. doi:10.1002/elsc.201600241
Abstract: The growing demand for t-resveratrol for industrial uses has generated considerable interest in its production. Heterologous resveratrol production in plant cell suspensions, apart from requiring the introduction of only one or two genes, has the advantage of high biomass yield and a short cultivation time, and thus could be an option for large-scale production. Silybum marianum is the source of the flavonolignan silymarin. Phenylpropanoid synthesis in cultures of this species can be activated by elicitation with methyl jasmonate and methylated β-cyclodextrins, with products of the pathway (coniferyl alcohol and some isomers of the silymarin complex) being released into the medium. Given that stilbene synthase shares the same key precursors involved in flavonoid and /or monolignol biosynthesis, we explored the potential of metabolically engineered S. marianum cultures for t-resveratrol production. Cell suspensions were stably transformed with Vitis vinifera stilbene synthase 3 and the expression of the transgene led to extracellular t-resveratrol accumulation at the level of milligrams per litre under elicitation. Resveratrol synthesis occurred at the expense of coniferyl alcohol. Production of silymarin was less affected in the transgenic cultures, since the flavonoid pathway is limiting for its synthesis, due to the preferred supply of precursors for the monolignol branch. The fact that the expressed STS gene took excessively produced precursors of non-bioactive compounds (coniferyl alcohol), while keeping the metabolic flow for target secondary compounds (i.e. silymarin) unaltered, opens a way to extend the applications of plant cell cultures for the simultaneous production of both constitutive and foreign valuable metabolites.
Sponsor: This work has been supported by a grant from the Spanish Ministry of Science and Innovation (BIO2014-51861-R). Diego Hidalgo is a predoctoral fellow of Mexican CONACYT.
URI: http://hdl.handle.net/10045/67478
ISSN: 1618-0240 (Print) | 1618-2863 (Online)
DOI: 10.1002/elsc.201600241
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Peer Review: si
Publisher version: http://dx.doi.org/10.1002/elsc.201600241
Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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